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Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was supported by a grant from the Spanish Ministry of Economy and Competitiveness (PID2019-105838RB-C31). We thank the support of the scholarship of David Fernandez Polo from Spanish Ministry of Education through the National Program FPU (FPU18/01866).

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Enzymatic bioconversion of beechwood xylan into the antioxidant 2 '-O-alpha-(4-O-methyl-D-glucuronosyl)-xylobiose

Publicado en:Biomass Conversion and Biorefinery. - 2022-09-07 (), DOI: 10.1007/s13399-022-03240-3

Autores: Miguez, Noa; Fernandez-Polo, David; Santos-Moriano, Paloma; Rodriguez-Colinas, Barbara; Poveda, Ana; Jimenez-Barbero, Jesus; Ballesteros, Antonio O.; Plou, Francisco J.;

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Acidic xylooligosaccharides (XOS), also called aldouronics, are hetero-oligomers of xylose randomly branched with 4-O-methyl-D-glucuronic acid residues linked by alpha(1 -> 2) bonds, which display bioactive properties. We have developed a simple and integrated method for the production of acidic XOS by enzymatic hydrolysis of a glucurono-xylan from beechwood. Among the enzymes screened, Depol 670L (a cellulolytic preparation from Trichoderma reesei) displayed the highest activity (70.3 U/mL, expressed in reducing xylose equivalents). High-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) analysis revealed the formation of a neutral fraction (corresponding to linear XOS, mainly xylose and xylobiose) and a group of more retained products (acidic XOS), which were separated using strong anion-exchange cartridges. The acidic fraction contained a major product, characterized by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry and mono- and two-dimensional nuclear magnetic resonance spectroscopy (NMR) as 2 '-O-alpha-(4-O-methyl-alpha-D-glucuronosyl)-xylobiose (X2_MeGlcA). Starting from 2 g of beechwood xylan, 1.5 g of total XOS were obtained, from which 225 mg (11% yield) corresponded to the aldouronic X2_MeGlcA. The acidic XOS exhibited higher antioxidant activity (measured by the ABTS(center dot+) discoloration assay) than xylan, whilst neutral XOS displayed no antioxidant activity. This work demonstrates that it is possible to obtain a safe and natural antioxidant by enzymatic biotransformation of hardwood hemicellulose.

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