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Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was supported by a grant from the Spanish Ministry of Economy and Competitiveness (PID2019-105838RB-C31). We thank the support of the scholarship of David Fernandez Polo from Spanish Ministry of Education through the National Program FPU (FPU18/01866).

Analysis of institutional authors

Miguez, NoaAuthor

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Article

Enzymatic bioconversion of beechwood xylan into the antioxidant 2 '-O-alpha-(4-O-methyl-D-glucuronosyl)-xylobiose

Publicated to:Biomass Conversion and Biorefinery. - 2022-09-07 (), DOI: 10.1007/s13399-022-03240-3

Authors: Miguez, Noa; Fernandez-Polo, David; Santos-Moriano, Paloma; Rodriguez-Colinas, Barbara; Poveda, Ana; Jimenez-Barbero, Jesus; Ballesteros, Antonio O.; Plou, Francisco J.;

Affiliations

Basque Fdn Sci, Bilbao 48009, Spain - Author
Basque Res & Technol Alliance BRTA, CIC bioGUNE, Derio 48160, Spain - Author
CSIC, Inst Catalisis & Petroleoquim, Madrid 28049, Spain - Author

Abstract

Acidic xylooligosaccharides (XOS), also called aldouronics, are hetero-oligomers of xylose randomly branched with 4-O-methyl-D-glucuronic acid residues linked by alpha(1 -> 2) bonds, which display bioactive properties. We have developed a simple and integrated method for the production of acidic XOS by enzymatic hydrolysis of a glucurono-xylan from beechwood. Among the enzymes screened, Depol 670L (a cellulolytic preparation from Trichoderma reesei) displayed the highest activity (70.3 U/mL, expressed in reducing xylose equivalents). High-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) analysis revealed the formation of a neutral fraction (corresponding to linear XOS, mainly xylose and xylobiose) and a group of more retained products (acidic XOS), which were separated using strong anion-exchange cartridges. The acidic fraction contained a major product, characterized by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry and mono- and two-dimensional nuclear magnetic resonance spectroscopy (NMR) as 2 '-O-alpha-(4-O-methyl-alpha-D-glucuronosyl)-xylobiose (X2_MeGlcA). Starting from 2 g of beechwood xylan, 1.5 g of total XOS were obtained, from which 225 mg (11% yield) corresponded to the aldouronic X2_MeGlcA. The acidic XOS exhibited higher antioxidant activity (measured by the ABTS(center dot+) discoloration assay) than xylan, whilst neutral XOS displayed no antioxidant activity. This work demonstrates that it is possible to obtain a safe and natural antioxidant by enzymatic biotransformation of hardwood hemicellulose.

Keywords

Acidic xylooligosaccharidesAldouronic acidsAntioxidantsArabinoxylansBioactive oligosaccharidesEndoxylanaseHemicelluloseOligosaccharidesPrebioticsRiceWheatXylooligosaccharides

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Biomass Conversion and Biorefinery due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2022, it was in position 52/142, thus managing to position itself as a Q2 (Segundo Cuartil), in the category Engineering, Chemical. Notably, the journal is positioned en el Cuartil Q3 para la agencia Scopus (SJR) en la categoría Renewable Energy, Sustainability and the Environment.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-07-01:

  • WoS: 1
  • Scopus: 3

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-01:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 10.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 10 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.5.
  • The number of mentions on the social network X (formerly Twitter): 2 (Altmetric).

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (MIGUEZ RODRIGUEZ, NOA) .