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This research was supported by CONACYT (Mexico) grants 302979 and A1-S-9005 to R.M.D.A. However, the funders had no role in study design, data collection, analysis, publication decision, or manuscript preparation. The APC (Article Processing Charge) was partially funded by the PAIPC (Programa de apoyo e incentivos a publicaciones cientifcas, acronyms in Spanish) program of CONFIE (Coordinacion General para el Fomento a la Investigacion Cientifca e Innovacion del Estado de Sinaloa) and Sociedad Mexicana de Virologia (SMV).

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Antiviral Effect of Microalgae Phaeodactylum tricornutum Protein Hydrolysates against Dengue Virus Serotype 2

Publicado en:Marine Drugs. 22 (8): 369- - 2024-08-01 22(8), DOI: 10.3390/md22080369

Autores: Rivera-Serrano, Bianca Vianey; Cabanillas-Salcido, Sandy Lucero; Cordero-Rivera, Carlos Daniel; Jimenez-Camacho, Ricardo; Norzagaray-Valenzuela, Claudia Desiree; Calderon-Zamora, Loranda; De Jesus-Gonzalez, Luis Adrian; Reyes-Ruiz, Jose Manuel; Farfan-Morales, Carlos Noe; Romero-Utrilla, Alejandra; Ruiz-Ruelas, Victor Manuel; Camberos-Barraza, Josue; Camacho-Zamora, Alejandro; De la Herran-Arita, Alberto Kousuke; Angulo-Rojo, Carla; Guadron-Llanos, Alma Marlene; Rabago-Monzon, Angel Radames; Perales-Sanchez, Janitzio Xiomara Korina; Valdez-Flores, Marco Antonio; Del Angel, Rosa Maria; Osuna-Ramos, Juan Fidel

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Dengue, caused by the dengue virus (DENV), is a global health threat transmitted by Aedes mosquitoes, resulting in 400 million cases annually. The disease ranges from mild to severe, with potential progression to hemorrhagic dengue. Current research is focused on natural antivirals due to challenges in vector control. This study evaluates the antiviral potential of peptides derived from the microalgae Phaeodactylum tricornutum, known for its bioactive compounds. Microalgae were cultivated under controlled conditions, followed by protein extraction and hydrolysis to produce four peptide fractions. These fractions were assessed for cytotoxicity via the MTT assay and antiviral activity against DENV serotype 2 using flow cytometry and plaque formation assays. The 10-30 kDa peptide fraction, at 150 and 300 mu g/mL concentrations, demonstrated no cytotoxicity and significantly reduced the percentage of infected cells and viral titers. These findings suggest that peptides derived from Phaeodactylum tricornutum exhibit promising antiviral activity against dengue virus serotype 2, potentially contributing to developing new therapeutic approaches for dengue.

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