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Grant support

This research was conducted with the financial support of Division de Investigacion y Extension-sede Bogota (DIEB), Universidad Nacional de Colombia (Project 41569: peptidos quimericos derivados de la Lactoferricina bovina y la Buforina: diseno, sintesis y evaluacion de su actividad antibacteriana).

Analysis of institutional authors

Insuasty Cepeda, Diego SebastianAuthor

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April 4, 2023
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Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology

Publicated to:MOLECULES. 24 (7): - 2019-03-28 24(7), DOI: 10.3390/molecules24071215

Authors: Insuasty Cepeda, Diego Sebastian; Pineda Castaneda, Hector Manuel; Rodriguez Mayor, Andrea Veronica; Garcia Castaneda, Javier Eduardo; Maldonado Villamil, Mauricio; Fierro Medina, Ricardo; Rivera Monroy, Zuly Jenny;

Affiliations

Univ Nacl Colombia, Dept Chem, Carrera 45 26-85,Bldg 451,Off 409, Bogota 11321, Colombia - Author
Univ Nacl Colombia, Dept Pharm, Carrera 45 26-85,Bldg 450, Bogota 11321, Colombia - Author

Abstract

A methodology was implemented for purifying peptides in one chromatographic run via solid-phase extraction (SPE), reverse phase mode (RP), and gradient elution, obtaining high-purity products with good yields. Crude peptides were analyzed by reverse phase high performance liquid chromatography and a new mathematical model based on its retention time was developed in order to predict the percentage of organic modifier in which the peptide will elute in RP-SPE. This information was used for designing the elution program of each molecule. It was possible to purify peptides with different physicochemical properties, showing that this method is versatile and requires low solvent consumption, making it the least polluting one. Reverse phase-SPE can easily be routinely implemented. It is an alternative to enrich and purified synthetic or natural molecules.

Keywords

gradient elutionpeptidepreparative purificationsolid phase extraction (spe)Gradient elutionPeptidePreparative purificationSolid phase extraction (spe)Solid phase peptide synthesis

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal MOLECULES due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2019, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Chemistry (Miscellaneous).

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 14, 2024)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Field Citation Ratio (FCR) from Dimensions: 3.49 (source consulted: Dimensions Jul 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-07-09, the following number of citations:

  • WoS: 23

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-09:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 113.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 124 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.1.
  • The number of mentions on the social network X (formerly Twitter): 2 (Altmetric).

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Colombia.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (CEPEDA GOMEZ, DIEGO) .