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Analysis of institutional authors

Marin F.r.Corresponding AuthorSoler-Rivas C.Author
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RNA-Seq, Bioinformatic Identification of Potential MicroRNA-Like Small RNAs in the Edible Mushroom Agaricus bisporus and Experimental Approach for Their Validation

Publicated to:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 23 (9): 4923- - 2022-05-01 23(9), DOI: 10.3390/ijms23094923

Authors: Marin FR; Dávalos A; Kiltschewskij D; Crespo MC; Cairns M; Andrés-León E; Soler-Rivas C

Affiliations

Campus de Excelencia Internacional UAM+CSIC - Author
CSIC - Instituto de Parasitologia y Biomedicina Lopez Neyra (IPBLN) - Author
CSIC, Inst Parasitol & Biomed Lopez Neyra, Bioinformat Unit, Granada 18016, Spain - Author
Pabellon Cent Antiguo Hosp Cantoblanco, Madrid Inst Adv Studies IMDEA Food CEI UAM CSIC, Lab Epigenet Lipid Metab, Madrid 28049, Spain - Author
Univ Autonoma Madrid, Inst Food Sci Res CIAL UAM CSIC, Dept Prod & Characterizat Novel Foods, Madrid 28049, Spain - Author
Univ Newcastle, Fac Hlth & Med, Sch Biomed Sci & Pharm, Callaghan, NSW 2308, Australia - Author
Universidad Autónoma de Madrid - Author
University of Newcastle, Faculty of Health and Medicine - Author
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Abstract

Although genomes from many edible mushrooms are sequenced, studies on fungal micro RNAs (miRNAs) are scarce. Most of the bioinformatic tools are designed for plants or animals, but the processing and expression of fungal miRNAs share similarities and differences with both kingdoms. Moreover, since mushroom species such as Agaricus bisporus (A. bisporus, white button mushroom) are frequently consumed as food, controversial discussions are still evaluating whether their miRNAs might or might not be assimilated, perhaps within extracellular vesicles (i.e., exosomes). Therefore, the A. bisporus RNA-seq was studied in order to identify potential de novo miRNA-like small RNAs (milRNAs) that might allow their later detection in diet. Results pointed to 1 already known and 37 de novo milRNAs. Three milRNAs were selected for RT-qPCR experiments. Precursors and mature milRNAs were found in the edible parts (caps and stipes), validating the predictions carried out in silico. When their potential gene targets were investigated, results pointed that most were involved in primary and secondary metabolic regulation. However, when the human transcriptome is used as the target, the results suggest that they might interfere with important biological processes related with cancer, infection and neurodegenerative diseases.

Keywords
agaricus bisporuscellsfungigenomemilrnasmirbasemirnamirnasoriginspathwaysrt-qpcrsequencetargetstoolwhite button mushroomAgaricus bisporusFungiGene-expressionMilrnasMirnasRt-qpcrWhite button mushroom

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2022, it was in position 66/285, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations from Scopus Elsevier, it yields a value for the Field-Weighted Citation Impact from the Scopus agency: 1.03, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 14, 2024)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Field Citation Ratio (FCR) from Dimensions: 2.56 (source consulted: Dimensions May 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-05-02, the following number of citations:

  • WoS: 3
  • Scopus: 6
  • Europe PMC: 4
  • OpenCitations: 6
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-02:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 29.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 29 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 5.2.
  • The number of mentions on the social network Facebook: 1 (Altmetric).
  • The number of mentions on the social network X (formerly Twitter): 8 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/706005
Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Australia.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (MARIN MARTIN, FRANCISCO RAMON) and Last Author (SOLER RIVAS, CRISTINA).

the author responsible for correspondence tasks has been MARIN MARTIN, FRANCISCO RAMON.