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Grant support

This work has been funded by grant SAF2017-82886-R from the Spanish Ministry of Economy and Competitiveness (MINECO), grant S2017/BMD-3671-INFLAMUNE-CM from the Comunidad de Madrid, a grant from the Ramo ' n Areces Foundation "Ciencias de la Vida y la Salud" (CIVP19A5941 XIX Concurso-2018), and a grant from Ayudas Fundacio ' n BBVA a Equipos de Investigacio ' n Cienti ' fica (BIOMEDICINA-2018), `La Caixa' Banking Foundation (HR17-00016) and grants BIOIMID (PIE13/041) and CIBER Cardiovascular (CB16/11/00272) from Fondo de Investigacio ' n Sanitaria del Instituto de Salud Carlos III and cofunding by Fondo Europeo de Desarrollo Regional (FEDER). The Centro Nacional de Investigaciones Cardiovasculares (CNIC) is supported by the Spanish Ministry of Economy and Competitiveness (MINECO) and the Pro-CNIC Foundation and is a Severo Ochoa Center of Excellence (MINECO award SEV-2015-0505). Authors declare no competing interest. Funding agencies have not participated in the design of the studies, with no copyright over the study.

Analysis of institutional authors

Sanz Sanz, JesusAuthorMartín-Cófreces NbCorresponding AuthorFernández-Gallego NAuthorSanchez-Madrid FAuthor

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March 26, 2021
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TIRF Microscopy as a Tool to Determine Exosome Composition

Publicated to:Methods in Molecular Biology. 2346 91-104 - 2021-01-01 2346(), DOI: 10.1007/7651_2020_320

Authors: Martin-Cofreces, Noa B; Torralba, Daniel; Lozano-Prieto, Marta; Fernandez-Gallego, Nieves; Sanchez-Madrid, Francisco

Affiliations

Centro de Investigación Biomédica en Red Cardiovascular (CIBERCV), Madrid, Spain. fsmadrid@salud.madrid.org. - Author
Centro de Investigación Biomédica en Red Cardiovascular (CIBERCV), Madrid, Spain. noa.martin@salud.madrid.org. - Author
Ctr Invest Biomed Red Cardiovasc CIBERCV, Madrid, Spain - Author
Ctr Nacl Invest Cardiovasc CNIC, Vasc Pathophysiol Area, Madrid, Spain - Author
Servicio de Inmunología. Hospital Universitario de la Princesa, Universidad Autónoma de Madrid, Instituto Investigación Sanitaria Princesa (IIS-IP), Madrid, Spain. - Author
Servicio de Inmunología. Hospital Universitario de la Princesa, Universidad Autónoma de Madrid, Instituto Investigación Sanitaria Princesa (IIS-IP), Madrid, Spain. fsmadrid@salud.madrid.org. - Author
Servicio de Inmunología. Hospital Universitario de la Princesa, Universidad Autónoma de Madrid, Instituto Investigación Sanitaria Princesa (IIS-IP), Madrid, Spain. noa.martin@salud.madrid.org. - Author
Univ Autonoma Madrid, Inst Invest Sanitaria Princesa IIS IP, Hosp Univ Princesa, Serv Inmunol, Madrid, Spain - Author
Vascular Pathophysiology Area. Centro Nacional Investigaciones Cardiovasculares (CNIC), Madrid, Spain. - Author
Vascular Pathophysiology Area. Centro Nacional Investigaciones Cardiovasculares (CNIC), Madrid, Spain. fsmadrid@salud.madrid.org. - Author
Vascular Pathophysiology Area. Centro Nacional Investigaciones Cardiovasculares (CNIC), Madrid, Spain. noa.martin@salud.madrid.org. - Author
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Abstract

Exosomes are extracellular vesicles (EVs) containing different biomolecules with biological activity, such as proteins, miRNA, long noncoding RNA, and DNA. EVs are efficient platforms for intercellular communication, especially during immune responses, but also in some pathological contexts, such as tumor cell growth. The precise assessment of EV content is relevant for the selection of specific vesicles with specialized biological activities, whose content is hardly visualized due to their small size. We describe herein a protocol for the determination of the content of individual EVs through microscopy imaging and user-friendly analysis using TIRF microscopy.

Keywords

cell-to-cell communicationexosomesimmune synapsemicroscopyt cellstirfCell-to-cell communicationExosomesExtracellular vesiclesImmune synapseMicroscopyT cellsTirfVesicles

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Methods in Molecular Biology, Q4 Agency Scopus (SJR), its regional focus and specialization in Genetics, give it significant recognition in a specific niche of scientific knowledge at an international level.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 1.1, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jul 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-07-16, the following number of citations:

  • WoS: 5
  • Scopus: 7
  • Europe PMC: 2

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-16:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 26 (PlumX).

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (MARTIN COFRECES, NOA BEATRIZ) and Last Author (SANCHEZ MADRID, FRANCISCO).

the author responsible for correspondence tasks has been MARTIN COFRECES, NOA BEATRIZ.