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Grant support

This work was funded by grants from the CICyT (MAT2006-12603-CO2-O2), Comunidad de Madrid (S-0505/MAT/0324), and from the CONSOLIDER-INGENIO program (FUNCOAT-CSD2008-00023). DMM was funded by a grant from the Fundacion Conchita Rabago de Jimenez Diaz.

Analysis of institutional authors

Cordero Ampuero, JoseAuthorFernandez Roblas, RicardoAuthorGomez Barrena, EnriqueAuthorEsteban Moreno, JaimeAuthor
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Article

Biofilm development by clinical isolates of Staphylococcus spp. from retrieved orthopedic prostheses

Publicated to:Acta Orthopaedica. 81 (6): 674-679 - 2010-12-01 81(6), DOI: 10.3109/17453674.2010.537810

Authors: Esteban, Jaime; Molina-Manso, Diana; Spiliopoulou, Iris; Cordero-Ampuero, Jose; Fernandez-Roblas, Ricardo; Foka, Antigoni; Gomez-Barrena, Enrique;

Affiliations

University of Patras, School of Medicine - Author
‎ Fdn Jimenez Diaz UTE, Dept Clin Microbiol, Madrid, Spain - Author
‎ Fdn Jimenez Diaz UTE, Dept Orthopaed, Madrid, Spain - Author
‎ Hosp Univ La Princesa, Dept Orthopaed, Madrid, Spain - Author
‎ Univ Patras, Sch Med, Dept Microbiol, GR-26110 Patras, Greece - Author
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Abstract

Background Biofilms are considered the key factor in the development of implant-related infections. However, only a few reports have dealt with the ability of organisms isolated from such infections to develop biofilms in vitro. Methods We evaluated different phenotypic techniques (2 microtiter plate assays and confocal laser scanning microscopy (CLSM) and genotypic techniques (detection of the ica operon) related to biofilm development by clinical isolates of Staphylococcus spp. Results All 26 strains tested (from 23 specimens) were biofilm producers. Stepanovic test detected biofilm formation in 85% of the strains, microtiter plate assay in 65%, and CLSM in 39%. The ica operon was detected in 73% of all strains (all 13 S. aureus strains and 6 of the 13 coagulase-negative Staphylococcus strains). 7 ica-negative strains were biofilm-positive by phenotypic methods. Interpretation The detection of ica genes could not be related to the phenotypic ability of the strains to develop a biofilm in vitro, so both studies (genetic and phenotypic) are required for a better evaluation of the biofilm-producing ability of clinical strains of Staphylococcus isolated from orthopedic infections.

Keywords
AdherenceAureusDiagnosisEpidermidisImplantsInfectionsPlates

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Acta Orthopaedica due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2010, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Medicine (Miscellaneous). Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 5.95, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions May 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-05-02, the following number of citations:

  • WoS: 42
  • Scopus: 47
  • Europe PMC: 35
  • Google Scholar: 80
  • OpenCitations: 49
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-02:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 65 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Greece.