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Grant support

This study was supported by grants to the PI, R.B., from the Ministerio de Economia, Industria y Competitividad (MEIC: SAF2013-44329-P, SAF2013-42359-ERC and RYC-2013-13209) and the European Research Council (ERC-2014-StG - 638028 AngioGenesHD). M.F. was supported by a PhD fellowship from Fundacion La Caixa (CX_E-201501) and W.L. by a FP7-PEOPLE-2012-COFUND GA600396 postdoctoral contract. We thank J.L. Pompa, G. Sabio, V. Andres, J.M. Redondo, F. Radtke, G. Breier, S. Martin, R.H. Adams, M. Torres, T. Schimmang and T. Honjo for sharing the Tie2-Cre, Alb-Cre, Ubc-CreERT2, MyHC-Cre, Dll4 and Notch1 floxed, Kdr floxed, Epas1 floxed, Cdh5(PAC)-CreERT2, Myc floxed, Mycn floxed and Rbpj floxed mice, respectively. We thank Simon Bartlett for English editing; Sofia Sanchez for help with the mouse colony; and all members of the CNIC gene targeting, transgenesis, cellomics, and microscopy units. The CNIC is supported by the Ministerio de Ciencia, Innovacion y Universidades (MCNU) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015-0505).

Analysis of institutional authors

Fernandez-Chacon, MacarenaAuthor

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Article

iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications

Publicated to:Nature Communications. 10 (2262): 2262- - 2019-12-01 10(2262), DOI: 10.1038/s41467-019-10239-4

Authors: Fernandez-Chacon, Macarena; Casquero-Garcia, Veronica; Luo, Wen; Francesca Lunella, Federica; Ferreira Rocha, Susana; Del Olmo-Cabrera, Sergio; Benedito, Rui;

Affiliations

CNIC, Mol Genet Angiogenesis Grp, E-28029 Madrid, Spain - Author

Abstract

Most biomedical research aimed at understanding gene function uses the Cre-Lox system, which consists of the Cre recombinase-dependent deletion of genes containing LoxP sites. This system enables conditional genetic modifications because the expression and activity of the recombinase Cre/CreERT2 can be regulated in space by tissue-specific promoters and in time by the ligand tamoxifen. Since the precise Cre-Lox recombination event is invisible, methods were developed to report Cre activity and are widely used. However, numerous studies have shown that expression of a given Cre activity reporter cannot be assumed to indicate deletion of other LoxP-flanked genes of interest. Here, we report the generation of an inducible dual reporter-Cre mouse allele, iSuRe-Cre. By significantly increasing Cre activity in reporter-expressing cells, iSuRe-Cre provides certainty that these cells have completely recombined floxed alleles. This genetic tool increases the ease, efficiency, and reliability of conditional mutagenesis and gene function analysis.

Keywords

CellsEfficiencyExpressionInhibitionKnockoutLigandLoxpMycRecombinationTransgenic mice

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Nature Communications due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2019, it was in position 6/71, thus managing to position itself as a Q1 (Primer Cuartil), in the category Multidisciplinary Sciences. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations from Scopus Elsevier, it yields a value for the Field-Weighted Citation Impact from the Scopus agency: 1.27, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 14, 2024)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Field Citation Ratio (FCR) from Dimensions: 6.15 (source consulted: Dimensions Jun 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-30, the following number of citations:

  • WoS: 19
  • Scopus: 33
  • Europe PMC: 21

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-30:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 145.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 145 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 34.6.
  • The number of mentions on the social network X (formerly Twitter): 29 (Altmetric).
  • The number of mentions in news outlets: 2 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (FERNANDEZ CHACON, MACARENA) .