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We dedicate this work to our colleagues and friends Bernd Fischer and Katrin Eichelbaum, who sadly passed away during the development of this work. We thank Matthias W. Hentze, Encarnacion Martinez-Salas, Javier Martinez, Kui Li, Quentin Sattentau, Ilan Davis, Richard Parton, and Jan Rehwinkel for reagents and advice. We thank the Oxford Micron Advanced Bioimaging Unit for support in microscopy experiments. A.C. is funded by MRC Career Development Award MR/L019434/1, MRC grant MR/R021562/1, and John Fell Funds from the University of Oxford. M.G.M. is funded by the European Union's Horizon 2020 research and innovation programme under Marie-Sklodowska-Curie grant agreement 700184. I.D. is funded by Wellcome Trust Investigator Award 209412/Z/17/Z.V.P. is funded by a SciLifeLab Fellowship, the Swedish Research Council (VR 2016-01842), a Wallenberg Academy Fellowship (KAW 2016.0123), and the Ragnar Soderberg Foundation. L.C. is funded by grant DGICYT SAF2015-66170-R (MINECO/FEDER). E.G.A. was awarded with a short-term EMBO fellowship (ASTF 358-2015) and an FPU fellowship FPU15/05709. G.M. was supported by the Wellcome Seed Award in Science (210144/Z/18/Z) and the Wellcome Trust Centre Core Grant (092076). G.H. was a recipient of a Wellcome Trust PhD studentship (105246/Z/14/Z).

Analysis of institutional authors

Garcia-Moreno, ManuelAuthorGonzalez-Almela, EstherAuthorCarrasco, LuisAuthor

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Article

System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection

Publicated to:MOLECULAR CELL. 74 (1): 196-+ - 2019-04-04 74(1), DOI: 10.1016/j.molcel.2019.01.017

Authors: Garcia-Moreno, Manuel; Noerenberg, Marko; Ni, Shuai; Jarvelin, Aino, I; Gonzalez-Almela, Esther; Lenz, Caroline E.; Bach-Pages, Marcel; Cox, Victoria; Avolio, Rosario; Davis, Thomas; Hester, Svenja; Sohier, Thibault J. M.; Li, Bingnan; Heikel, Gregory; Michlewski, Gracjan; Sanz, Miguel A.; Carrasco, Luis; Ricci, Emiliano P.; Pelechano, Vicent; Davis, Ilan; Fischer, Bernd; Mohammed, Shabaz; Castello, Alfredo;

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Abstract

The compendium of RNA-binding proteins (RBPs) has been greatly expanded by the development of RNA-interactome capture (RIC). However, it remained unknown if the complement of RBPs changes in response to environmental perturbations and whether these rearrangements are important. To answer these questions, we developed comparative RIC and applied it to cells challenged with an RNA virus called sindbis (SINV). Over 200 RBPs display differential interaction with RNA upon SINV infection. These alterations are mainly driven by the loss of cellular mRNAs and the emergence of viral RNA. RBPs stimulated by the infection redistribute to viral replication factories and regulate the capacity of the virus to infect. For example, ablation of XRN1 causes cells to be refractory to SINV, while GEMIN5 moonlights as a regulator of SINV gene expression. In summary, RNA availability controls RBP localization and function in SINV-infected cells.

Keywords

alphavirusgemin5host-virus interactionprotein-rna interactionrna-binding proteinrna-interactome capturesindbistrim25xrn1AlphavirusGemin5GeneHost-virus interactionIdentificationLigaseMessenger-rnaProtein-rna interactionProteomeRecognitionRna-binding proteinRna-interactome captureSindbisSmn complexStructural insightsTranslationTrim25Virus infectionXrn1

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal MOLECULAR CELL due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2019, it was in position 4/297, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 4. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 14, 2024)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 5.23 (source consulted: FECYT Feb 2024)
  • Field Citation Ratio (FCR) from Dimensions: 28.22 (source consulted: Dimensions May 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-05-29, the following number of citations:

  • WoS: 92
  • Scopus: 135
  • Europe PMC: 89
  • Google Scholar: 156
  • OpenCitations: 146

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-29:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 319.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 316 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 71.13.
  • The number of mentions on the social network X (formerly Twitter): 92 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/718879

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: China; France; Germany; Italy; Sweden; United Kingdom.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (GARCIA MORENO, MANUEL) .