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Grant support

This work was supported by grants from the Spanish Ministry of Economy, Industry and Competitiveness (Grants BIO2016-76601-C3-1-R and BIO2016-76601-C3-3-R). We thank the support of COST-Action CM1303 on Systems Biocatalysis. M.G.-P. thanks the Spanish Ministry of Education for FPU Grant. We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).

Analysis of institutional authors

Miguez, NoaAuthorGimeno-Perez, MariaAuthorFernandez-Lobato, MariaAuthor

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July 3, 2018
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Immobilization of the beta-fructofuranosidase from Xanthophyllomyces dendrorhous by Entrapment in Polyvinyl Alcohol and Its Application to Neo-Fructooligosaccharides Production

Publicated to:Catalysts. 8 (5): 201- - 2018-05-01 8(5), DOI: 10.3390/catal8050201

Authors: Miguez, Noa; Gimeno-Perez, Maria; Fernandez-Polo, David; Cervantes, Fadia V; Ballesteros, Antonio O; Fernandez-Lobato, Maria; Ribeiro, Maria H; Plou, Francisco J

Affiliations

CSIC UAM, Ctr Biol Mol Severo Ochoa, Madrid 28049, Spain - Author
CSIC, Inst Catalisis & Petr Quim, E-28049 Madrid, Spain - Author
Univ Lisbon, Res Inst Med iMed ULisboa, Fac Farm, P-1649003 Lisbon, Portugal - Author

Abstract

The beta-fructofuranosidase (Xd-INV) from the basidiomycota yeast Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma) is unique in its ability to synthesize neo- fructooligosaccharides (neo-FOS). In order to facilitate its industrial application, the recombinant enzyme expressed in Pichia pastoris (pXd-INV) was immobilized by entrapment in polyvinyl alcohol (PVA) hydrogels. The encapsulation efficiency exceeded 80%. The PVA lenticular particles of immobilized pXd-INV were stable up to approximately 40 degrees C. Using 600 g/L sucrose, the immobilized biocatalyst synthesized 18.9% (w/w) FOS (59.1 g/L of neokestose, 30.2 g/L of 1-kestose, 11.6 g/L of neonystose and 12.6 g/L of blastose). The operational stability of PVA-immobilized biocatalyst was assayed in a batch reactor at 30 degrees C. The enzyme preserved its initial activity during at least 7 cycles of 26 h.

Keywords

Aspergillus-aculeatusBioreactorsDextransucraseEnzyme entrapmentEnzyme immobilizationFructooligosaccharidesFructosyltransferaseGlucooligosaccharidesGlycosidasesHuman colonic microbiotaHydrogelsImmobilizationNanoparticlesNeokestoseOligosaccharidesOperational stabilityPrebioticsTransfructosylating activity

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Catalysts due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2018, it was in position 56/146, thus managing to position itself as a Q2 (Segundo Cuartil), in the category Chemistry, Physical. Notably, the journal is positioned en el Cuartil Q2 para la agencia Scopus (SJR) en la categoría Physical and Theoretical Chemistry.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 1.97, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Aug 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-08-28, the following number of citations:

  • WoS: 17
  • Scopus: 18

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-28:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 29.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 29 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 0.5.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Portugal.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (MIGUEZ RODRIGUEZ, NOA) .