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Funding was provided by Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT) 1140504 (http://www.conicyt.cl/fondecyt/); Mejoramiento de la Calidad y la Equidad en la Educacion Superior (MECESUP) 2-UCH0604 (http://www.mecesup.cl/) NB; and Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) 21110252 (http://www.conicyt.cl/becas-conicyt/) PC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Fernandez-Lobato, MAuthor

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December 2, 2016
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The Involvement of Mig1 from Xanthophyllomyces dendrorhous in Catabolic Repression: An Active Mechanism Contributing to the Regulation of Carotenoid Production

Publicated to:PLoS ONE. 11 (9): e0162838- - 2016-09-13 11(9), DOI: 10.1371/journal.pone.0162838

Authors: Alcaino, Jennifer; Bravo, Natalia; Cordova, Pamela; Marcoleta, Andres E; Contreras, Gabriela; Barahona, Salvador; Sepulveda, Dionisia; Fernandez-Lobato, Maria; Baeza, Marcelo; Cifuentes, Victor

Affiliations

Univ Autonoma Madrid, CSIC, Dept Biol Mol, Ctr Biol Mol Severo Ochoa, Madrid, Cantoblanco, Spain - Author
Univ Chile, Fac Ciencias, Dept Biol, Santiago, Chile - Author
Univ Chile, Fac Ciencias, Dept Ciencias Ecol, Lab Genet, Santiago, Chile - Author

Abstract

The red yeast X. dendrorhous is one of the few natural sources of astaxanthin, a carotenoid used in aquaculture for salmonid fish pigmentation and in the cosmetic and pharmaceutical industries for its antioxidant properties. Genetic control of carotenogenesis is well characterized in this yeast; however, little is known about the regulation of the carotenogenesis process. Several lines of evidence have suggested that carotenogenesis is regulated by catabolic repression, and the aim of this work was to identify and functionally characterize the X. dendrorhous MIG1 gene encoding the catabolic repressor Mig1, which mediates transcriptional glucose-dependent repression in other yeasts and fungi. The identified gene encodes a protein of 863 amino acids that demonstrates the characteristic conserved features of Mig1 proteins, and binds in vitro to DNA fragments containing Mig1 boxes. Gene functionality was demonstrated by heterologous complementation in a S. cerevisiae mig1- strain; several aspects of catabolic repression were restored by the X. dendrorhous MIG1 gene. Additionally, a X. dendrorhous mig1- mutant was constructed and demonstrated a higher carotenoid content than the wild-type strain. Most important, the mig1- mutation alleviated the glucose-mediated repression of carotenogenesis in X. dendrorhous: the addition of glucose to mig1- and wild-type cultures promoted the growth of both strains, but carotenoid synthesis was observed only in the mutant strain. Transcriptomic and RT-qPCR analyses revealed that several genes were differentially expressed between X. dendrorhous mig1- and the wild-type strain when cultured with glucose as the sole carbon source. The results obtained in this study demonstrate that catabolic repression in X. dendrorhous is an active process in which the identified MIG1 gene product plays a central role in the regulation of several biological processes, including carotenogenesis.

Keywords

Aspergillus-nidulansAstaxanthin biosynthesis pathwayBeta-caroteneFunctional-characterizationGene-expressionGlucose repressionPhaffia-rhodozymaSynthase geneTranscriptional regulationYeast saccharomyces-cerevisiae

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal PLoS ONE due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2016, it was in position 15/63, thus managing to position itself as a Q1 (Primer Cuartil), in the category Multidisciplinary Sciences.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations from Scopus Elsevier, it yields a value for the Field-Weighted Citation Impact from the Scopus agency: 1.36, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 14, 2024)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Field Citation Ratio (FCR) from Dimensions: 2.59 (source consulted: Dimensions Jul 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-07-03, the following number of citations:

  • WoS: 20
  • Scopus: 25
  • Europe PMC: 13

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-03:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 32.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 32 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 10.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Chile.