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Carrasco, EAuthorCalvo, M IAuthorBlazquez-Castro, AAuthorZamarron, AAuthorJuarranz, AAuthorEspada, JCorresponding Author

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November 6, 2015
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Photoactivation of ROS Production In Situ Transiently Activates Cell Proliferation in Mouse Skin and in the Hair Follicle Stem Cell Niche Promoting Hair Growth and Wound Healing

Publicated to:JOURNAL OF INVESTIGATIVE DERMATOLOGY. 135 (11): 2611-2622 - 2015-11-01 135(11), DOI: 10.1038/jid.2015.248

Authors: Carrasco, Elisa; Calvo, Maria I; Blazquez-Castro, Alfonso; Vecchio, Daniela; Zamarron, Alicia; Joyce Dias de Almeida, Irma; Stockert, Juan C; Hamblin, Michael R; Juarranz, Angeles; Espada, Jesus

Affiliations

1] Instituto de Investigaciones Biomedicas 'Alberto Sols', Departamento de Bioquimica, CSIC-Universidad Autonoma de Madrid, Madrid, Spain [2] Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Madrid, Spain - Author
1] Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA [2] Department of Dermatology, Harvard Medical School, Boston, Massachusetts, USA - Author
Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Madrid, Spain - Author
Harvard Univ, Sch Med, Dept Dermatol, Boston, MA 02115 USA - Author
Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 USA - Author
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA - Author
Univ Autonoma Madrid, Dept Bioquim, CSIC, Inst Invest Biomed Alberto Sols, E-28049 Madrid, Spain - Author
Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, Spain - Author
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Abstract

The role of reactive oxygen species (ROS) in the regulation of hair follicle (HF) cycle and skin homeostasis is poorly characterized. ROS have been traditionally linked to human disease and aging, but recent findings suggest that they can also have beneficial physiological functions in vivo in mammals. To test this hypothesis, we transiently switched on in situ ROS production in mouse skin. This process activated cell proliferation in the tissue and, interestingly, in the bulge region of the HF, a major reservoir of epidermal stem cells, promoting hair growth, as well as stimulating tissue repair after severe burn injury. We further show that these effects were associated with a transient Src kinase phosphorylation at Tyr416 and with a strong transcriptional activation of the prolactin family 2 subfamily c of growth factors. Our results point to potentially relevant modes of skin homeostasis regulation and demonstrate that a local and transient ROS production can regulate stem cell and tissue function in the whole organism.

Keywords

BiochemistryBiodiversidadeBiotecnologíaCell biologyCiências biológicas iCiências biológicas iiCiências biológicas iiiDermatologyDermatology & venereal diseasesEducação físicaEngenharias iiFarmaciaGeneral medicineInterdisciplinarMedicina iMedicina iiMedicina iiiMolecular biologyOdontología

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal JOURNAL OF INVESTIGATIVE DERMATOLOGY due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2015, it was in position 1/61, thus managing to position itself as a Q1 (Primer Cuartil), in the category Dermatology.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 3.42. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 14, 2024)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 2.12 (source consulted: FECYT Feb 2024)
  • Field Citation Ratio (FCR) from Dimensions: 7.6 (source consulted: Dimensions Jul 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-07-03, the following number of citations:

  • WoS: 64
  • Scopus: 69
  • Europe PMC: 43

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-03:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 82.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 82 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 18.5.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).
  • The number of mentions in news outlets: 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Denmark; United States of America.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (CARRASCO CERRO, ELISA) and Last Author (ESPADA REGALADO, JESUS).

the author responsible for correspondence tasks has been ESPADA REGALADO, JESUS.