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Analysis of institutional authors

Garrido-Sanz, DanielCorresponding Author

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October 30, 2025
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Expanding the Pseudomonas diversity of the wheat rhizosphere: four novel species antagonizing fungal phytopathogens and with plant-beneficial properties

Publicated to: Frontiers In Microbiology. 15 1440341- - 2024-07-15 15(), DOI: 10.3389/fmicb.2024.1440341

Authors:

Poli, Noemie; Keel, Christoph Joseph; Garrido-Sanz, Daniel
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Affiliations

Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland. - Author
Univ Lausanne, Dept Fundamental Microbiol, Lausanne, Switzerland - Author

Abstract

Plant-beneficial Pseudomonas bacteria hold the potential to be used as inoculants in agriculture to promote plant growth and health through various mechanisms. The discovery of new strains tailored to specific agricultural needs remains an open area of research. In this study, we report the isolation and characterization of four novel Pseudomonas species associated with the wheat rhizosphere. Comparative genomic analysis with all available Pseudomonas type strains revealed species-level differences, substantiated by both digital DNA-DNA hybridization and average nucleotide identity, underscoring their status as novel species. This was further validated by the phenotypic differences observed when compared to their closest relatives. Three of the novel species belong to the P. fluorescens species complex, with two representing a novel lineage in the Pseudomonas phylogeny. Functional genome annotation revealed the presence of specific features contributing to rhizosphere colonization, including flagella and components for biofilm formation. The novel species have the genetic potential to solubilize nutrients by acidifying the environment, releasing alkaline phosphatases and their metabolism of nitrogen species, indicating potential as biofertilizers. Additionally, the novel species possess traits that may facilitate direct promotion of plant growth through the modulation of the plant hormone balance, including the ACC deaminase enzyme and auxin metabolism. The presence of biosynthetic clusters for toxins such as hydrogen cyanide and non-ribosomal peptides suggests their ability to compete with other microorganisms, including plant pathogens. Direct inoculation of wheat roots significantly enhanced plant growth, with two strains doubling shoot biomass. Three of the strains effectively antagonized fungal phytopathogens (Thielaviopsis basicola, Fusarium oxysporum, and Botrytis cinerea), demonstrating their potential as biocontrol agents. Based on the observed genetic and phenotypic differences from closely related species, we propose the following names for the four novel species: Pseudomonas grandcourensis sp. nov., type strain DGS24(T) ( = DSM 117501(T) = CECT 31011(T)), Pseudomonas purpurea sp. nov., type strain DGS26(T) ( = DSM 117502(T )= CECT 31012(T)), Pseudomonas helvetica sp. nov., type strain DGS28(T) ( = DSM 117503(T) = CECT 31013(T)) and Pseudomonas aestiva sp. nov., type strain DGS32(T) ( = DSM 117504(T) = CECT 31014(T)).
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Keywords

DiversityFungal phytopathogen antagonismNovel speciesPhylogenomcisPlant-beneficial bacteriaPseudomonasRhizosphereWheat

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Frontiers in Microbiology due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 38/163, thus managing to position itself as a Q1 (Primer Cuartil), in the category Microbiology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-05:

  • WoS: 7
  • Scopus: 7
  • Europe PMC: 6
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-05:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 31.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 31 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 17.
  • The number of mentions on the social network X (formerly Twitter): 11 (Altmetric).
  • The number of mentions on Wikipedia: 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Switzerland.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (GARRIDO SANZ, DANIEL).

the author responsible for correspondence tasks has been GARRIDO SANZ, DANIEL.

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Awards linked to the item

We thank the Genomic Technologies Facility of the University of Lausanne, Switzerland, for sequencing the genomes of the four reported strains, the Electron Microscopy Facility of the University of Lausanne for the help with preparation of samples and their imaging, and Caterina Matasci (Delley Seeds and Plants Ltd., Switzerland), for providing us with the wheat seeds used in this study.
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