{rfName}
Ti

Indexed in

License and use

Icono OpenAccess

Citations

10

Altmetrics

Analysis of institutional authors

García-García P.Corresponding AuthorSeñorans F.j.Author

Share

Publications
>
Article

Tisochrysis lutea as a source of omega-3 polar lipids and fucoxanthin: extraction and characterization using green solvents and advanced techniques

Publicated to:JOURNAL OF APPLIED PHYCOLOGY. 36 (4): 1697-1708 - 2024-08-01 36(4), DOI: 10.1007/s10811-024-03233-x

Authors: García-García P; Ospina M; Señoráns FJ

Affiliations

Universidad Autónoma de Madrid - Author

Abstract

The marine microalga Tisochrysis lutea is a potential and sustainable source of bioactive compounds such as carotenoids and omega-3 fatty acids. In the present work, the extraction of fucoxanthin and docosahexaenoic acid (DHA), the most abundant omega-3 fatty acid which constitutes polar lipids particularly in the brain, was studied using advanced extraction techniques with green and bio-based solvents compared to traditional extraction techniques with hazardous organic solvents. The experimental design to maximize the lipid extraction yield by ultrasound-assisted extraction (UAE) was developed, choosing as experimental factors the percentage of solvent (0, 50 and 100% of 2-methyl-tetrahydrofuran or 2-methyloxolane (2-me-THF) in ethanol), the extraction time (20, 30 and 40 min) and temperature (40, 50 and 60 ºC). The highest lipid extraction yields were obtained using ethanol as solvent. Nevertheless, the most interesting extracts based on their chemical composition were obtained when the presence of 2-me-THF in the extraction mixture was greater than that of ethanol. Through analytical techniques such as HPLC-ELSD/DAD and GC-MS as well as spectrophotometric techniques, the contents of polar lipids, fatty acids, total carotenoids and fucoxanthin were quantified. In addition, the antioxidant capacity of different selected extracts was studied, being once again the most interesting those extracted with different amounts of 2-me-THF due to its selectivity and enriched composition in high-added value bioactives, mainly fucoxanthin and DHA. Therefore, it is shown the importance of choosing an advanced extraction technique together with the use of green solvents not only to develop procedures that are in agreement with Green Chemistry but also to preserve its bioactivity.

Keywords

Aquatic scienceBiodiversidadeBiotechnology & applied microbiologyBiotecnologíaCiência de alimentosCiências agrárias iCiências ambientaisCiências biológicas iCiências biológicas iiCiências biológicas iiiEnfermagemEngenharias iiEngenharias iiiFarmaciaGeociênciasInterdisciplinarMarine & freshwater biologyMedicina iMedicina iiPlant sciencePsicologíaQuímicaZootecnia / recursos pesqueiros

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal JOURNAL OF APPLIED PHYCOLOGY due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 88/174, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biotechnology & Applied Microbiology.

This publication has been distinguished as a “Highly Cited Paper” by the agencies WoS (ESI, Clarivate) and ESI (Clarivate), meaning that it ranks within the top 1% of the most cited articles in its thematic field during the year of its publication. In terms of the observed impact of the contribution, this work is considered one of the most influential worldwide, as it is recognized as highly cited. (source consulted: ESI Nov 14, 2024)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-16, the following number of citations:

  • WoS: 4
  • Scopus: 6

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-16:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 27 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

    It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

    • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

    Leadership analysis of institutional authors

    There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (GARCIA GARCIA, MARIA PAZ) and Last Author (SEÑORANS RODRIGUEZ, FCO.JAVIER).

    the author responsible for correspondence tasks has been GARCIA GARCIA, MARIA PAZ.