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This work has been possible thanks to funding allocated to ED from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Individual Fellowship grant agreement No. 796084 (qMAR). After qMAR termination, ED has been funded from the Agencia Estatal de Investigacion of the Spanish Ministry of Science and Innovation in the form of a Juan de la Cierva - Incorporacion grant number IJC2019-038832-I. Follow-up work has been allowed by support provided by Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, grants PI16/01209 and PI19/01356 to JM. Co-financed by European Development Regional Fund "A way to achieve Europe". The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Intestinal loads of extended-spectrum beta-lactamase and Carbapenemase genes in critically ill pediatric patients

Publicado en:Frontiers in Cellular and Infection Microbiology. 13 1180714- - 2023-05-02 13(), DOI: 10.3389/fcimb.2023.1180714

Autores: Dahdouh, Elias; Cendejas-Bueno, Emilio; Ruiz-Carrascoso, Guillermo; Schuffelmann, Cristina; Lazaro-Perona, Fernando; Castro-Martinez, Mercedes; Moreno-Ramos, Francisco; Escosa-Garcia, Luis; Alguacil-Guillen, Marina; Mingorance, Jesus

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Introduction: Intestinal colonization by Multi-Drug Resistant Organisms (MDROs) can pose a threat on the health of critically ill patients. The extent of colonization by these organisms is related to previous antibiotic treatments and their ability to cause infections among adult patients. The aim of this study is to determine the relationship between the intestinal Relative Loads (RLs) of selected antibiotic resistance genes, antibiotic consumption and extra-intestinal spread among critically ill pediatric patients. Methods: RLs of bla(CTX-M-1-Family), bla(OXA-1), bla(OXA-48) and bla(VIM) were determined in 382 rectal swabs obtained from 90 pediatric critically ill patients using qPCRs. The RLs were compared to the patients' demographics, antibiotic consumption, and detection of MDROs from extra-intestinal sites. 16SrDNA metagenomic sequencing was performed for 40 samples and clonality analyses were done for representative isolates. Results and discussion: 76 (74.45%) patients from which 340 (89.01%) rectal swabs were collected had at least one swab that was positive for one of the tested genes. Routine cultures did not identify carbapenemases in 32 (45.1%) and 78 (58.2%) swabs that were positive by PCR for bla (OXA-48) and blaVIM, respectively. RLs of above 6.5% were associated with extra-intestinal spread of blaOXA-48-harboring MDROs. Consumption of carbapenems, non-carbapenem beta-lactams, and glycopeptides were statistically associated with testing negative for bla (CTX-M-1-Family) and bla (OXA-1) while the consumption of trimethoprim/sulfamethoxazole and aminoglycosides was associated with testing negative for blaOXA-48 (P

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