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The authors thank Morote translation services for its assistance with the language editing and HULP-IdiPAZ Biobank for sample processing and the cell culture facility at IdiPAZ. We also thank the financial support.

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Diestro, Maria DoloresAuthor

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MiR-151a: a robust endogenous control for normalizing small extracellular vesicle cargo in human cancer

Publicated to:Biomark Res. 11 (1): - 2023-10-20 11(1), DOI: 10.1186/s40364-023-00526-0

Authors: Burdiel, M; Jiménez, J; Rodríguez-Antolín, C; García-Guede, A; Pernía, O; Sastre-Perona, A; Rosas-Alonso, R; Colmenarejo, J; Rodríguez-Jiménez, C; Diestro, MD; Martínez-Marín, V; Higueras, O; Cruz, P; Losantos-García, I; Peinado, H; Vera, O; de Castro, J; de Cáceres, II

Affiliations

IdiPAZ, Biomarkers & Expt Therapeut Canc, Madrid, Spain - Author
Paz Univ Hosp, Gynecol Oncol Unit, Madrid, Spain - Author
Paz Univ Hosp, IdiPAZ, Biostat Unit, Madrid, Spain - Author
Paz Univ Hosp, INGEMM, Canc Epigenet Lab, Madrid, Spain - Author
Paz Univ Hosp, Med Oncol Dept, Madrid, Spain - Author
Spanish Natl Canc Res Ctr CNIO, Mol Oncol Programme, Microenvironm & Metastasis Lab, Madrid, Spain - Author
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Abstract

Small extracellular vesicles (sEVs) in the blood of cancer patients contain higher amounts of tumor markers than those identified as free-circulating. miRNAs have significant biomedical relevance due to their high stability and feasible detection. However, there is no reliable endogenous control available to measure sEVs-miRNA content, impairing the acquisition of standardized consistent measurements in cancer liquid biopsy. In this study, we identified three miRNAs from a panel of nine potential normalizers that emerged from a comprehensive analysis comparing the sEV-miRNA profile of six lung and ovarian human cancer cell lines in the absence of or under different conditions. Their relevance as normalizers was tested in 26 additional human cancer cell lines from nine different tumor types undergoing chemotherapy or radiotherapy treatment. The validation cohorts were comprised of 242 prospective plasma and ascitic fluid samples from three different human tumor types. Variability and normalization properties were tested in comparison to miR-16, the most used control to normalize free-circulating miRNAs in plasma. Our results indicate that miR-151a is consistently represented in small extracellular vesicles with minimal variability compared to miR-16, providing a novel normalizer to measure small extracellular vesicle miRNA content that will benefit liquid biopsy in cancer patients.

Keywords

Igfbp-3Mirna endogenous control in liquid biopsyMirnas standardizationSevs

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Biomark Res due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2023, it was in position 13/189, thus managing to position itself as a Q1 (Primer Cuartil), in the category Medicine, Research & Experimental. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 1.78, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jun 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-22, the following number of citations:

  • Scopus: 2

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-22:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 10.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 10 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 10.45.
  • The number of mentions on the social network X (formerly Twitter): 10 (Altmetric).