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Grant support

This work has been supported by Comunidad Autonoma de Madrid (project No. S2009/PPQ-1642), Comunidad Autonoma de Madrid/Universidad Autonoma de Madrid (projects No. CCG07-UAM/SEM-1542 and CCG08-UAM/SEM-4074) and Ministerio de Ciencia e Innovacion (projects No. CTQ2008-05775, FIS2009-12964-C05-04 and Consolider No. CSD2007-00010).

Analysis of institutional authors

Casero Junquera, Maria ElenaAuthorGismera Garcia, Maria JesusAuthorPariente Alonso, FelixAuthorParra Alfambra, Ana MariaAuthorPetit Dominguez, Maria DoloresAuthorLorenzo, EAuthor

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Article

One-step covalent microcontact printing approach to produce patterns of lactate oxidase

Publicated to:PHYSICAL CHEMISTRY CHEMICAL PHYSICS. 12 (12): 2830-2837 - 2010-03-19 12(12), DOI: 10.1039/b922805e

Authors: Casero, E.; Petit-Dominguez, M. D.; Parra-Alfambra, A. M.; Gismera, M. J.; Pariente, F.; Lorenzo, E.; Vazquez, L.;

Affiliations

CSIC, Inst Ciencia Mat, E-28049 Madrid, Spain - Author
Univ Autonoma Madrid, Dept Quim Analit & Anal Instrumental, E-28049 Madrid, Spain - Author
Universidad Autónoma de Madrid - Author

Abstract

A comparative study of three different strategies to pattern lactate oxidase (LOx) onto bare gold substrates by microcontact printing (mu CP) is presented. The quality of the resulting patterns in terms of homogeneity, compactness and stability has been evaluated by atomic force microscopy in both air and aqueous conditions. The following approaches have been tested: (i) LOx was directly stamped to a bare gold surface; (ii) LOx was previously covalently bonded to a thiolated molecule, dithiodipropionic acid di(N-succinimidyl ester) (DTSP), and this conjugate (LOx/DTSP) was transferred from an elastomeric stamp to a bare gold substrate; (iii) formation of a LOx/DTSP micropattern on a bare gold surface (as described in approach ii) was followed by exposure to a solution containing hexadecylmercaptane (HDM). In all cases, the catalytic activity of the final LOx patterns has been assessed by electrochemical measurements. From comparison of the three strategies, it can be concluded that the third one gives rise to LOx patterns that present a high stability and compactness, also offering the advantage of reducing the number of microcontact printing steps to one.

Keywords

ArraysFabricationGoldMicrometerNanostructuresProteinsScanning electrochemical microscopySelf-assembled monolayersSoft-lithographySurfaces

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal PHYSICAL CHEMISTRY CHEMICAL PHYSICS due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2010, it was in position 5/33, thus managing to position itself as a Q1 (Primer Cuartil), in the category Physics, Atomic, Molecular & Chemical.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-06-24:

  • Google Scholar: 8
  • WoS: 6
  • Scopus: 6
  • Europe PMC: 4
  • OpenCitations: 6

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-24:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 12 (PlumX).

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Casero, E) .

the author responsible for correspondence tasks has been Casero, E.