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Analysis of institutional authors

Fresno Escudero, ManuelAuthorGalán-Martínez JAuthorBerenguer IAuthorStamatakis KAuthorGironès NAuthorFresno MCorresponding Author

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July 11, 2022
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Article

TCFL5 deficiency impairs the pachytene to diplotene transition during spermatogenesis in the mouse

Publicated to:Scientific Reports. 12 (1): 10956- - 2022-12-01 12(1), DOI: 10.1038/s41598-022-15167-w

Authors: Galan-Martinez, Javier; Berenguer, Ines; del Carmen Maza, Ma; Stamatakis, Konstantinos; Girones, Nuria; Fresno, Manuel

Affiliations

CSIC, Madrid, Spain - Author
CSIC-UAM - Centro de Biología Molecular Severo Ochoa (CBM) , Consejo Superior de Investigaciones Científicas - Author
Ctr Biol Mol Severo Ochoa, Nicolas Cabrera 1, Madrid 28049, Spain - Author
Inst Sanitario Invest Princesa, Madrid, Spain - Author
Univ Autonoma Madrid, Madrid, Spain - Author
Universidad Autónoma de Madrid , CSIC-UAM - Centro de Biología Molecular Severo Ochoa (CBM) - Author
Universidad Autónoma de Madrid , CSIC-UAM - Centro de Biología Molecular Severo Ochoa (CBM) , Instituto Sanitario de Investigación Princesa - Author
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Abstract

Spermatogenesis is a complex, multistep process during which spermatogonia give rise to spermatozoa. Transcription Factor Like 5 (TCFL5) is a transcription factor that has been described expressed during spermatogenesis. In order to decipher the role of TCFL5 during in vivo spermatogenesis, we generated two mouse models. Ubiquitous removal of TCFL5 generated by breeding TCFL5fl/fl with SOX2-Cre mice resulted in sterile males being unable to produce spermatozoa due to a dramatic alteration of the testis architecture presenting meiosis arrest and lack of spermatids. SYCP3, SYCP1 and H1T expression analysis showed that TCFL5 deficiency causes alterations during pachytene/diplotene transition resulting in a meiotic arrest in a diplotene-like stage. Even more, TCFL5 deficient pachytene showed alterations in the number of MLH1 foci and the condensation of the sexual body. In addition, tamoxifen-inducible TCFL5 knockout mice showed, besides meiosis phenotype, alterations in the spermatids elongation process resulting in aberrant spermatids. Furthermore, TCFL5 deficiency increased spermatogonia maintenance genes (Dalz, Sox2, and Dmrt1) but also increased meiosis genes (Syce1, Stag3, and Morc2a) suggesting that the synaptonemal complex forms well, but cannot separate and meiosis does not proceed. TCFL5 is able to bind to the promoter of Syce1, Stag3, Dmrt1, and Syce1 suggesting a direct control of their expression. In conclusion, TCFL5 plays an essential role in spermatogenesis progression being indispensable for meiosis resolution and spermatids maturation.

Keywords

calmegincomplexgenemale-infertilitymanchettemeiosisproteinrecombinationtranscription factorAnimalsBasic helix-loop-helix transcription factorsCell cycle proteinsCell self-renewalMaleMeiosisMiceMice, knockoutSpermatocytesSpermatogenesisTcfl5 protein, mouseTestisTranscription factors

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Scientific Reports due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2022, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Multidisciplinary. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.72. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 14, 2024)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 1.59 (source consulted: FECYT Feb 2024)
  • Field Citation Ratio (FCR) from Dimensions: 10.8 (source consulted: Dimensions Jul 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-07-04, the following number of citations:

  • WoS: 10
  • Scopus: 10
  • Europe PMC: 9

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-04:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 7.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 7 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.25.
  • The number of mentions on the social network X (formerly Twitter): 2 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/706510

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (GALAN MARTINEZ, JAVIER) and Last Author (FRESNO GARCIA, MARIA TERESA).

the author responsible for correspondence tasks has been FRESNO GARCIA, MARIA TERESA.