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Grant support

This research was supported by Fundacio Marato TV3 (110533), Catalunya, Spain, Comision Sectorial de Investigacion Cientifica (CSIC-UDELAR), Uruguay, PEDECIBA, Uruguay, Agencia Nacional de Investigacion e Innovacion (ANII), Uruguay, and FOCEM (MERCOSUR Structural Convergence Fund), COF 03/1111, CIBERNED, and TERCEL funds from the Instituto de Salud Carlos III of Spain. The authors thank the technical help of Jessica Jaramillo, Marta Valeri, and Natalia Puig.

Analysis of institutional authors

Francos-Quijorna, IsaacAuthor
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Article

CD300f immunoreceptor contributes to peripheral nerve regeneration by the modulation of macrophage inflammatory phenotype

Publicated to:Journal of Neuroinflammation. 12 (1): 145- - 2015-08-12 12(1), DOI: 10.1186/s12974-015-0364-y

Authors: Peluffo, Hugo; Solari-Saquieres, Patricia; Luciana Negro-Demontel, Maria; Francos-Quijorna, Isaac; Navarro, Xavier; Lopez-Vales, Ruben; Sayos, Joan; Lago, Natalia;

Affiliations

Ctr Invest Biomed Red Enfermedades Neurodegenerat, Bellaterra, Spain - Author
Inst Pasteur Montevideo, Neurodegenerat Lab, Montevideo, Uruguay - Author
Inst Pasteur Montevideo, Neuroinflammat & Gene Therapy Lab, Montevideo 11400, Uruguay - Author
UDELAR, Dept Histol & Embryol, Fac Med, Montevideo, Uruguay - Author
Univ Autonoma Barcelona, Dept Cell Biol Physiol & Immunol, Bellaterra, Spain - Author
Univ Autonoma Barcelona, Inst Neurosci, Bellaterra, Spain - Author
Univ Autonoma Barcelona, Inst Recerca VHIR, Hosp Univ Vall dHebron, Immunobiol Grp,CIBBIM Nanomed Program, Barcelona, Spain - Author
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Abstract

Background: It has recently become evident that activating/inhibitory cell surface immune receptors play a critical role in regulating immune and inflammatory processes in the central nervous system (CNS). The immunoreceptor CD300f expressed on monocytes, neutrophils, and mast cells modulates inflammation, phagocytosis, and outcome in models of autoimmune demyelination, allergy, and systemic lupus erythematosus. On the other hand, a finely regulated inflammatory response is essential to induce regeneration after injury to peripheral nerves since hematogenous macrophages, together with resident macrophages and de-differentiated Schwann cells, phagocyte distal axonal and myelin debris in a well-orchestrated inflammatory response. The possible roles and expression of CD300f and its ligands have not been reported under these conditions.Methods: By using quantitative PCR (QPCR) and CD300f-IgG2a fusion protein, we show the expression of CD300f and its ligands in the normal and crush injured sciatic nerve. The putative role of CD300f in peripheral nerve regeneration was analyzed by blocking receptor-ligand interaction with the same CD300f-IgG2a soluble receptor fusion protein in sciatic nerves of Thy1-YFP-H mice injected at the time of injury. Macrophage M1/M2 polarization phenotype was also analyzed by CD206 and iNOS expression.Results: We found an upregulation of CD300f mRNA and protein expression after injury. Moreover, the ligands are present in restricted membrane patches of Schwann cells, which remain stable after the lesion. The lesioned sciatic nerves of Thy1-YFP-H mice injected with a single dose of CD300f-IgG2a show long lasting effects on nerve regeneration characterized by a lower number of YFP-positive fibres growing into the tibial nerve after 10 days post lesion (dpl) and a delayed functional recovery when compared to PBS-or IgG2a-administered control groups. Animals treated with CD300f-IgG2a show at 10 dpl higher numbers of macrophages and CD206-positive cells and lower levels of iNOS expression than both control groups. At later time points (28 dpl), increased numbers of macrophages and iNOS expression occur.Conclusions: Taken together, these results show that the pair CD300f ligand is implicated in Wallerian degeneration and nerve regeneration by modulating both the influx and phenotype of macrophages.

Keywords
Axon regenerationCd300ImmunoreceptorsInjuryMacrophage m1/m2 phenotypeMyelin-associated glycoproteinNecrosis-factor-alphaPhagocytosisRat-brainRegenerationSchwann cellSciatic-nerveSpatiotemporal quantificationSystemTnf-alphaWallerian degeneration

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Journal of Neuroinflammation due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2015, it was in position 50/256, thus managing to position itself as a Q1 (Primer Cuartil), in the category Neurosciences.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.04. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 14, 2024)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 1.05 (source consulted: FECYT Feb 2024)
  • Field Citation Ratio (FCR) from Dimensions: 6.54 (source consulted: Dimensions May 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-05-17, the following number of citations:

  • WoS: 37
  • Scopus: 43
  • OpenCitations: 38
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-17:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 92.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 92 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.25.
  • The number of mentions on the social network X (formerly Twitter): 3 (Altmetric).
Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Uruguay.