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Analysis of institutional authors

Diestro MdCorresponding AuthorZapardiel IAuthorSánchez-Pastor MAuthorHardisson DCorresponding Author

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October 26, 2021
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Article

One-step nucleic acid amplification (Osna) of sentinel lymph node in early-stage endometrial cancer: Spanish multicenter study (endo-osna)

Publicated to:Cancers. 13 (17): 4465- - 2021-09-01 13(17), DOI: 10.3390/cancers13174465

Authors: Diestro, Maria Dolores; Berjon, Alberto; Zapardiel, Ignacio; Yebenes, Laura; Ruiz, Irune; Lekuona, Arantza; Rezola, Marta; Jaunarena, Ibon; Siegrist, Jaime; Sanchez-Pastor, Margarita; Cuadra, Maria; Sagasta, Amaia; Guerra, Isabel; Lete, Luis, I; Roldan, Fernando; Marta, Carlo B; Boillos, Maria; Cardiel, Maria; Lopez-de la Manzanara, Carlos; Relea, Fernanda; Coronado, Pluvio; Pascual, Alejandro; Roman, Maria; Peiro, Gloria; Matute, Luis; Montero, Beatriz; Muruzabal, Juan; Guarch, Rosa; Zorrero, Cristina; Calatrava, Ana; Ribot, Laia; Costa, Irmgard; Hernandez, Alicia; Hardisson, David

Affiliations

Alicante Inst Hlth & Biomed Res ISABIAL, Alicante 03010, Spain - Author
Complejo Hosp Univ Navarra, Dept Gynecol, Pamplona 31008, Spain - Author
Complejo Hosp Univ Navarra, Dept Pathol, Pamplona 31008, Spain - Author
Corp Sanitaria Parc Tauli Sabadell, Dept Gynecol, Barcelona 08208, Spain - Author
Corp Sanitaria Parc Tauli Sabadell, Dept Pathol, Barcelona 08208, Spain - Author
Hosp Clin Univ Lozano Blesa, Dept Gynecol, Zaragoza 50009, Spain - Author
Hosp Clin Univ Lozano Blesa, Dept Pathol, Zaragoza 50009, Spain - Author
Hosp Clin Univ San Carlos, Dept Pathol, Madrid 28040, Spain - Author
Hosp Fdn IVO, Dept Gynecol, Valencia 46009, Spain - Author
Hosp Fdn IVO, Dept Pathol, Valencia 46009, Spain - Author
Hosp Gen Univ Alicante, Dept Gynecol, Alicante 03010, Spain - Author
Hosp Gen Univ Alicante, Dept Pathol, Alicante 03010, Spain - Author
Hosp Univ & Politecn La Fe, Dept Gynecol, Valencia 46026, Spain - Author
Hosp Univ & Politecn La Fe, Dept Pathol, Valencia 46026, Spain - Author
Hosp Univ Donostia Osakidetza, Dept Gynecol, Donostia San Sebastian 20014, Spain - Author
Hosp Univ Donostia Osakidetza, Dept Pathol, Donostia San Sebastian 20014, Spain - Author
OSI Araba Hosp Univ, Inst Invest Bioaraba, Dept Gynecol, Vitoria 01009, Spain - Author
OSI Araba Hosp Univ, Inst Invest Bioaraba, Dept Pathol, Vitoria 01009, Spain - Author
Univ Autonoma Madrid, Hosp Univ La Paz, Hosp La Paz Inst Hlth Res IdiPAZ, Fac Med,Gynecol Oncol Unit,Dept Gynecol & Obstet, Madrid 28046, Spain - Author
Univ Autonoma Madrid, Hosp Univ La Paz,CIBERONC,Fac Med, Hosp La Paz Inst Hlth Res IdiPAZ,Ctr Biomed Res C, Dept Pathol,Inst Salud Carlos III,Mol Pathol & Th, Madrid 28046, Spain - Author
Univ Castilla La Mancha, Hosp Gen Univ Ciudad Real, Dept Gynecol, Ciudad Real 13005, Spain - Author
Univ Castilla La Mancha, Hosp Gen Univ Ciudad Real, Dept Pathol, Ciudad Real 13005, Spain - Author
Univ Complutense Madrid, Hosp Clin Univ San Carlos, Women Hlth Inst, IdISSC, Madrid 28040, Spain - Author
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Abstract

The objective of this study was to evaluate the efficacy of one-step nucleic acid amplification (OSNA) for the detection of sentinel lymph node (SLN) metastasis compared to standard pathological ultrastaging in patients with early-stage endometrial cancer (EC). A total of 526 SLNs from 191 patients with EC were included in the study, and 379 SLNs (147 patients) were evaluated by both methods, OSNA and standard pathological ultrastaging. The central 1 mm portion of each lymph node was subjected to semi-serial sectioning at 200 µm intervals and examined by hematoxylin–eosin and immunohistochemistry with CK19; the remaining tissue was analyzed by OSNA for CK19 mRNA. The OSNA assay detected metastases in 19.7% of patients (14.9% micrometastasis and 4.8% macrometastasis), whereas pathological ultrastaging detected metastasis in 8.8% of patients (3.4% micrometastasis and 5.4% macrometastasis). Using the established cut-off value for detecting SLN metastasis by OSNA in EC (250 copies/µL), the sensitivity of the OSNA assay was 92%, specificity was 82%, diagnostic accuracy was 83%, and the negative predictive value was 99%. Discordant results between both methods were recorded in 20 patients (13.6%). OSNA resulted in an upstaging in 12 patients (8.2%). OSNA could aid in the identification of patients requiring adjuvant treatment at the time of diagnosis.

Keywords

cytokeratin 19endometrial cancermicrometastasesone-step nucleic acid amplificationosnasentinel lymph nodeCytokeratin 19Endometrial cancerMicrometastasesOne-step nucleic acid amplificationOsnaSentinel lymph nodeUltrastaging

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Cancers due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2021, it was in position 60/245, thus managing to position itself as a Q1 (Primer Cuartil), in the category Oncology.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 3.6, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jul 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-07-16, the following number of citations:

  • WoS: 11
  • Scopus: 10
  • Europe PMC: 4

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-16:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 23.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 22 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 4.8.
  • The number of mentions on the social network X (formerly Twitter): 7 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/716676

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (DIESTRO TEJEDA, MARIA DOLORES) and Last Author (HARDISSON HERNAEZ, DAVID ALONSO).

the authors responsible for correspondence tasks have been DIESTRO TEJEDA, MARIA DOLORES and HARDISSON HERNAEZ, DAVID ALONSO.