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Toribio VAuthorYanez-Mo MAuthor

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June 7, 2021
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Embryonic trophectoderm secretomics reveals chemotactic migration and intercellular communication of endometrial and circulating mscs in embryonic implantation

Publicated to:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 22 (11): - 2021-06-01 22(11), DOI: 10.3390/ijms22115638

Authors: Calle A; Toribio V; Yáñez-Mó M; Ramírez MÁ

Affiliations

CSIC-UAM - Centro de Biología Molecular Severo Ochoa (CBM) - Author
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria - Author
Universidad Autónoma de Madrid - Author
‎ Inst Invest Sanitaria Princesa IIS IP, CBM SO, Madrid 28049, Spain - Author
‎ Inst Nacl Invest & Tecnol Agr & Alimentaria INIA, Dept Reprod Anim, Madrid 28040, Spain - Author
‎ UAM, Dept Biol Mol, Madrid 28049, Spain - Author
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Abstract

Embryonic implantation is a key step in the establishment of pregnancy. In the present work, we have carried out an in-depth proteomic analysis of the secretome (extracellular vesicles and soluble proteins) of two bovine blastocysts embryonic trophectoderm primary cultures (BBT), confirming different epithelial–mesenchymal transition stages in these cells. BBT-secretomes contain early pregnancy-related proteins and angiogenic proteins both as cargo in EVs and the soluble fraction. We have demonstrated the functional transfer of protein-containing secretome between embryonic trophectoderm and maternal MSC in vitro using two BBT primary cultures eight endometrial MSC (eMSC) and five peripheral blood MSC (pbMSC) lines. We observed that eMSC and pbMSC chemotax to both the soluble fraction and EVs of the BBT secretome. In addition, in a complementary direction, we found that the pattern of expression of implantation proteins in BBT-EVs changes depending on: (i) their epithelial–mesenchymal phenotype; (ii) as a result of the uptake of eMSC-or pbMSC-EV previously stimulated or not with embryonic signals (IFN-τ); (iii) because of the stimulation with the endometrial cytokines present in the uterine fluid in the peri-implantation period.

Keywords

cell migrationepithelial to mesenchymal transitionextracellular vesiclesmesenchymal stromal cellsBovineCell migrationCell nuclear transferEpithelial to mesenchymal transitionEpithelial-mesenchymal transitionExtracellular vesiclesFollistatinGene-expressionIntegrin receptorsMesenchymal stromal cellsPregnancyTrophectoderm

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2021, it was in position 69/297, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 2.27, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jul 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-07-09, the following number of citations:

  • WoS: 11
  • Scopus: 12
  • Europe PMC: 10

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-09:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 16 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/704449