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This work was supported by the Spanish Ministries of Economy and Competitiveness (BIO2016-76601-C3-1/-2/-3) and of Science and Innovation (PID2019-105838RB-C3-1/-2/-3) as well as Fundacion Ramon Areces (XIX Call of Research Grants in Life and Materials Sciences). We appreciate Fundacion Ramon Areces for providing an institutional grant to the Centro de Biologia Molecular Severo Ochoa. D. Piedrabuena was a recipient of a doctoral fellowship from the Spanish Ministry of Education, Culture, and Sports (FPU014/01004). We thank the staff members of the Synchrotron Radiation Source at Barcelona (ALBA, Spain) for providing access and technical assistance at BL13-XALOC beamline. We are also grateful to Mrs. Asuncion Martin-Redondo for her technical support.

Analysis of institutional authors

Rodrigo-Frutos, DAuthorPiedrabuena, DAuthorMiguez, NAuthorFernandez-Lobato, MCorresponding Author

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Article

New insights into the molecular mechanism behind mannitol and erythritol fructosylation by beta-fructofuranosidase from Schwanniomyces occidentalis

Publicated to:Scientific Reports. 11 (1): 7158- - 2021-12-01 11(1), DOI: 10.1038/s41598-021-86568-6

Authors: Rodrigo-Frutos, David; Jimenez-Ortega, Elena; Piedrabuena, David; Ramirez-Escudero, Mercedes; Miguez, Noa; Plou, Francisco J; Sanz-Aparicio, Julia; Fernandez-Lobato, Maria

Affiliations

CSIC, Dept Cristalog & Biol Estruct, Inst Fis Quim Rocasolano, Serrano 119, Madrid 28006, Spain - Author
CSIC, Inst Catalisis & Petr Quim ICP, Madrid 28049, Spain - Author
Ctr Biol Mol Severo Ochoa CBMSO, Nicolas Cabrera 1, Madrid 28049, Spain - Author
UAM CSIC, Dept Biol Mol, Nicolas Cabrera 1, Madrid 28049, Spain - Author
Univ Autonoma Madrid, Fac Ciencias, Nicolas Cabrera 1, Madrid 28049, Spain - Author
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Abstract

The beta -fructofuranosidase from Schwanniomyces occidentalis (Ffase) is a useful biotechnological tool for the fructosylation of different acceptors to produce fructooligosaccharides (FOS) and fructo-conjugates. In this work, the structural determinants of Ffase involved in the transfructosylating reaction of the alditols mannitol and erythritol have been studied in detail. Complexes with fructosyl-erythritol or sucrose were analyzed by crystallography and the effect of mutational changes in positions Gln-176, Gln-228, and Asn-254 studied to explore their role in modulating this biocatalytic process. Interestingly, N254T variant enhanced the wild-type protein production of fructosyl-erythritol and FOS by similar to 30% and 48%, respectively. Moreover, it produced neokestose, which represented similar to 27% of total FOS, and yielded 31.8 g l(-1) blastose by using glucose as exclusive fructosyl-acceptor. Noteworthy, N254D and Q176E replacements turned the specificity of Ffase transferase activity towards the synthesis of the fructosylated polyols at the expense of FOS production, but without increasing the total reaction efficiency. The results presented here highlight the relevance of the pair Gln-228/Asn-254 for Ffase donor-sucrose binding and opens new windows of opportunity for optimizing the generation of fructosyl-derivatives by this enzyme enhancing its biotechnological applicability.

Keywords

Astronomia / físicaBiodiversidadeBiotecnologíaCiência da computaçãoCiência de alimentosCiências agrárias iCiências ambientaisCiências biológicas iCiências biológicas iiCiências biológicas iiiEconomiaEducaçãoEducação físicaEnfermagemEngenharias iiEngenharias iiiEngenharias ivFarmaciaGeociênciasGeografíaInterdisciplinarLetras / linguísticaMatemática / probabilidade e estatísticaMateriaisMedicina iMedicina iiMedicina iiiMedicina veterinariaMultidisciplinaryMultidisciplinary sciencesNutriçãoOdontologíaPsicologíaQuímicaSaúde coletivaZootecnia / recursos pesqueiros

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Scientific Reports due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2021, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Multidisciplinary. Notably, the journal is positioned above the 90th percentile.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-06-18:

  • Open Alex: 6
  • WoS: 6
  • Scopus: 6
  • OpenCitations: 6

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-18:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 10.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 10 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 0.5.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/702781

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (RODRIGO FRUTOS, DAVID) and Last Author (FERNANDEZ LOBATO, MARIA).

the author responsible for correspondence tasks has been FERNANDEZ LOBATO, MARIA.