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Fortes Alen, Jose RomanAuthorCasado SAuthor
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Expression of endothelial nitric oxide synthase in human peritoneal tissue: Regulation by Escherichia coli lipopolysaccharide

Publicated to:JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY. 11 (10): 1848-1856 - 2000-10-24 11(10), DOI: 10.1681/ASN.V11101848

Authors: Arriero M; Rodríguez-Feo J; Celdrán A; De Miguel L; González-Fernández F; Fortes J; Reyero A; Frieyro O; De La Pinta J; Franco A; Pastor C; Casado S; López-Farré A

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Hospital Universitario Fundacion Jimenez Diaz - Author

Abstract

Changes in the expression of endothelial nitric oxide synthase (eNOS) in the peritoneum could be involved in the peritoneal dysfunction associated with peritoneal inflammation. Demonstrated recently in bovine endothelial cells was the existence of cytosolic proteins that bind to the 3'-untranslated region (3'-UTR) of eNOS mRNA and could be implicated in eNOS mRNA stabilization. The present work demonstrates that eNOS protein is expressed in human endothelial and mesothelial peritoneal cells. Escherichia coli lipopolysaccharide shortened the half-life of eNOS message, reducing eNOS protein expression in peritoneal mesothelial and endothelial cells. Moreover, under basal conditions, human peritoneal samples expressed cytosolic proteins that bind to the 3'-UTR of eNOS mRNA. The cytosolic proteins that directly bind to 3'-UTR were identified as a 60-kD protein. After incubation of human peritoneal samples with lipopolysaccharide, the binding activity of the cytosolic 60-kD protein increased in a time-dependent manner. Studies are now necessary to determine the involvement of this 60-kD protein in the regulation of eNOS expression in peritoneal cells and particularly its involvement in the peritoneal dysfunction associated with inflammatory reactions.

Keywords
Ciências biológicas iCiências biológicas iiCiências biológicas iiiCritical care and intensive care medicineEngenharias ivEpidemiologyInterdisciplinarMedicina iMedicina iiMedicina iiiMedicine (all)Medicine (miscellaneous)NephrologySaúde coletivaTransplantationUrology & nephrology

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2000, it was in position 1/43, thus managing to position itself as a Q1 (Primer Cuartil), in the category Urology & Nephrology.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 3.85, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions May 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-05-20, the following number of citations:

  • WoS: 23
  • Scopus: 30
  • OpenCitations: 26
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-20:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 8 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.