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Analysis of institutional authors

Hidalgo AAuthorBerenguer JAuthorMencía MAuthor

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Article

A Modular Vector Toolkit with a Tailored Set of Thermosensors to Regulate Gene Expression in Thermus thermophilus

Publicated to:ACS Omega. 4 (11): 14626-14632 - 2019-09-10 4(11), DOI: 10.1021/acsomega.9b02107

Authors: Verdú C., Sanchez E., Ortega C., Hidalgo A., Berenguer J., Mencía M.

Affiliations

CSIC-UAM - Centro de Biología Molecular Severo Ochoa (CBM) - Author

Abstract

Copyright © 2019 American Chemical Society. Modular plasmid architectures have shown to be a very useful resource to standardize, build, share, and compare biological parts and functional vectors, and are being applied in an increasing number of microorganisms. Here, we present a modular plasmid toolkit for Thermus thermophilus, a species considered as a workhorse for biotechnology and a model for high-temperature biology. Apart from integrating improved versions of already existing parts, we have characterized specific promoters and developed a thermosensor-based palette that restricts the expression to Thermus and, at the same time, controls protein expression in this organism in a temperature-dependent manner. ©

Keywords

Directed evolutionEscherichia-coliGenomeIn-vivoPromoterRibosomal-rnaS-layerSelection markerSequenceTranscription

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal ACS Omega due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2019, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Chemical Engineering (Miscellaneous).

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 1.12, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jun 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-24, the following number of citations:

  • WoS: 7
  • Scopus: 10
  • Europe PMC: 6
  • Google Scholar: 10
  • OpenCitations: 11

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-24:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 39 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/690982

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Verdú C) and Last Author (MENCIA CABALLERO, MARIO).