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Analysis of institutional authors

Salazar Ayestarán NAuthorJuarranz AAuthor

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August 1, 2016
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The PARP inhibitor PJ-34 sensitizes cells to UVA-induced phototoxicity by a PARP independent mechanism

Publicated to:MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS. 790 31-40 - 2016-08-01 790(), DOI: 10.1016/j.mrfmmm.2016.07.001

Authors: Lakatos P., Heged?s C., Salazar Ayestarán N., Juarranz Á., Kövér K., Szabó É., Virág L.

Affiliations

Department of Biology, Faculty of Sciences, Universidad Autónoma of Madrid, Madrid, Spain - Author
Department of Dermatology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary - Author
Department of Inorganic and Analytical Chemistry, Faculty of Sciences, University of Debrecen, Debrecen, Hungary - Author
Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary - Author
MTA-DE Cell Biology and Signaling Research Group, Debrecen, Hungary - Author
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Abstract

A combination of a photosensitizer with light of matching wavelength is a common treatment modality in various diseases including psoriasis, atopic dermatitis and tumors. DNA damage and production of reactive oxygen intermediates may impact pathological cellular functions and viability. Here we set out to investigate the role of the nuclear DNA nick sensor enzyme poly(ADP-ribose) polymerase 1 in photochemical treatment (PCT)-induced tumor cell killing. We found that silencing PARP-1 or inhibition of its enzymatic activity with Veliparib had no significant effect on the viability of A431 cells exposed to 8-methoxypsoralen (8-MOP) and UVA (2.5J/cm(2)) indicating that PARP-1 is not likely to be a key player in either cell survival or cell death of PCT-exposed cells. Interestingly, however, another commonly used PARP inhibitor PJ-34 proved to be a photosensitizer with potency equal to 8-MOP. Irradiation of PJ-34 with UVA caused changes both in the UV absorption and in the 1H NMR spectra of the compound with the latter suggesting UVA-induced formation of tautomeric forms of the compound. Characterization of the photosensitizing effect revealed that PJ-34+UVA triggers overproduction of reactive oxygen species, induces DNA damage, activation of caspase 3 and caspase 8 and internucleosomal DNA fragmentation. Cell death in this model could not be prevented by antioxidants (ascorbic acid, trolox, glutathione, gallotannin or cell permeable superoxide dismutase or catalase) but could be suppressed by inhibitors of caspase-3 and -8. In conclusion, PJ-34 is a photosensitizer and PJ-34+UVA causes DNA damage and caspase-mediated cell death independently of PARP-1 inhibition.Copyright © 2016 Elsevier B.V. All rights reserved.

Keywords

apoptosiscell deathpoly(adp-ribose) polymerase-1poly(adp-ribosyl)ationspheroidApoptosisCell deathPoly(adp-ribose) polymerase-1Poly(adp-ribosyl)ationSpheroidUltraviolet light

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2016, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Health, Toxicology and Mutagenesis.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-08-02:

  • WoS: 4
  • Scopus: 4

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-02:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 20 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Hungary.