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Gomez Lencero, RocioAuthorParra Catalan, Maria TeresaAuthorSanchez Rufas, JulioAuthorSuja Sanchez, Jose AngelAuthorViera Vicario, AlbertoAuthor

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Sequential Assembly of Centromeric Proteins in Male Mouse Meiosis

Publicated to:PLoS Genetics. 5 (3): e1000417-e1000417 - 2009-03-01 5(3), DOI: 10.1371/journal.pgen.1000417

Authors: Teresa Parra, Maria; Gomez, Rocio; Viera, Alberto; Llano, Elena; Pendas, Alberto M; Rufas, Julio S; Suja, Jose A

Affiliations

CSIC USAL, Inst Biol Mol & Celular Canc, Salamanca, Spain - Author
Departamento de Biología, Unidad de Biología Celular, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain. mayte.parra@uam.es - Author
Dept Fisiol, Salamanca, Spain - Author
Univ Autonoma Madrid, Fac Ciencias, Unidad Biol Celular, Dept Biol, E-28049 Madrid, Spain - Author

Abstract

The assembly of the mitotic centromere has been extensively studied in recent years, revealing the sequence and regulation of protein loading to this chromosome domain. However, few studies have analyzed centromere assembly during mammalian meiosis. This study specifically targets this approach on mouse spermatocytes. We have found that during prophase I, the proteins of the chromosomal passenger complex Borealin, INCENP, and Aurora-B load sequentially to the inner centromere before Shugoshin 2 and MCAK. The last proteins to be assembled are the outer kinetochore proteins BubR1 and CENP-E. All these proteins are not detected at the centromere during anaphase/telophase I and are then reloaded during interkinesis. The loading sequence of the analyzed proteins is similar during prophase I and interkinesis. These findings demonstrate that the interkinesis stage, regularly overlooked, is essential for centromere and kinetochore maturation and reorganization previous to the second meiotic division. We also demonstrate that Shugoshin 2 is necessary for the loading of MCAK at the inner centromere, but is dispensable for the loading of the outer kinetochore proteins BubR1 and CENP-E.

Keywords

Antropologia / arqueologiaAstronomia / físicaBiodiversidadeBiotecnologíaCancer researchCiência da computaçãoCiências agrárias iCiências biológicas iCiências biológicas iiCiências biológicas iiiEcology, evolution, behavior and systematicsEducação físicaEngenharias ivFarmaciaFilosofia/teologia:subcomissão filosofiaGeneticsGenetics & heredityGenetics (clinical)GeociênciasInterdisciplinarMedicina iMedicina iiMedicina iiiMedicina veterinariaMolecular biologyOdontologíaQuímicaSaúde coletivaZootecnia / recursos pesqueiros

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal PLoS Genetics due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2009, it was in position 10/146, thus managing to position itself as a Q1 (Primer Cuartil), in the category Genetics & Heredity.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 3.44, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jun 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-30, the following number of citations:

  • WoS: 39
  • Scopus: 42
  • Europe PMC: 33
  • Google Scholar: 48

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-30:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 67 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/13914