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Analysis of institutional authors

Martínez-Ortega JAuthorAlmendral JmAuthorStamatakis KAuthor

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April 1, 2026
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Effects of Multifrequency Electromagnetic Pulses on tumor growth in immunocompetent mice.

Publicated to: Scientific Reports. - 2026-04-01, DOI: 10.1038/s41598-026-49151-5

Authors:

Piredda R; Rodríguez Martínez LG; Martinez-Ortega J; López Ferraz A; Villatoro-Gómez S; Martín-García E; García-Bermejo ML; Almendral JM; Stamatakis K; Revilla Y
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Affiliations

Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, 28049, Madrid, Spain. - Author
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, 28049, Madrid, Spain. k.s@csic.es.; Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain. k.s@csic.es. - Author
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, 28049, Madrid, Spain. yrevilla@cbm.csic.es. - Author
Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain. - Author
Paso Alto Biotechnology INC, Miami, FL, USA. - Author
Universidad de La Laguna, Santa Cruz de Tenerife, Spain. - Author
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Abstract

We previously established that a Multifrequency Electromagnetic Pulse (MEMP) treatment selectively eradicates tumorigenic cells in vitro. Here we evaluate the effect of two different systemic MEMP treatment regimens (30 Hz, > 2T) on syngeneic MC38 colon adenocarcinoma subcutaneously engrafted in C57BL/6 mice. Tumor development was monitored by bioluminescence imaging, and excised tissues were evaluated for immune-cell infiltration (CD4, CD8, and CD68), oxidative stress (HO-1, MDA), and DNA damage (γH2AX) markers by immunohistochemistry. The MEMP regimes consistently and significantly slowed tumor progression and extended animal survival, with no detectable adverse effects on welfare. Histopathology revealed in treated tumors broader necrotic regions, signals of oxidative stress, and γH2AX staining showing limited yet evident DNA damage. While the number of intratumor CD4 and CD8 T-cell-counts remained stable, an apparent increase in the number CD68+ macrophages was noticed in the tumors of treated mice, suggesting that MEMP may enhance the penetration of immune effectors cells into the xenografted tumors, a preliminary evidence deserving further research. Further, MEMP achieved complete tumor regression in a small subset of animals, (n = 2), which mounted a robust resistance to a subsequent tumor re-challenge. Collectively, our findings indicate that systemic MEMP can alter tumor dynamics and immune features in pre-clinical animal models, offering a practical framework to study tumor–immune interactions at the organismal level under controlled physical-field conditions.
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Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Scientific Reports due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2026, it was in position 21/141, thus managing to position itself as a Q1 (Primer Cuartil), in the category Multidisciplinary Sciences.

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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-11-06:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 1.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 1 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 11.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).
  • The number of mentions in news outlets: 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: United States of America.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Piredda R) and Last Author (Revilla Y).

the author responsible for correspondence tasks has been Revilla Y.

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