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Impact on the Sustainable Development Goals (SDGs)

Analysis of institutional authors

Terreros-Roncal, JAuthorLlorens-Martin, MCorresponding Author

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January 5, 2026
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Prolonged fixation and post-mortem delay impede the study of adult neurogenesis in mice

Publicated to: Communications Biology. 6 (1): 978- - 2023-09-23 6(1), DOI: 10.1038/s42003-023-05367-z

Authors:

Gallardo-Caballero, M; Rodríguez-Moreno, CB; Alvarez-Méndez, L; Terreros-Roncal, J; Flor-García, M; Moreno-Jiménez, EP; Rábano, A; Llorens-Martín, M
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Affiliations

CIEN Fdn - Author
Ctr Networked Biomed Res Neurodegenerat Dis CIBERN - Author
Univ Autonoma Madrid, Fac Sci, Dept Mol Biol - Author
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Abstract

Adult hippocampal neurogenesis (AHN) gives rise to new neurons throughout life. This phenomenon takes place in more than 120 mammalian species, including humans, yet its occurrence in the latter was questioned after one study proposed the putative absence of neurogenesis markers in the adult human hippocampus. In this regard, we showed that prolonged fixation impedes the visualization of Doublecortin+ immature neurons in this structure, whereas other authors have suggested that a dilated post-mortem delay (PMD) underlies these discrepancies. Nevertheless, the individual and/or additive contribution of fixation and the PMD to the detection (or lack thereof) of other AHN markers has not been studied to date. To address this pivotal question, we used a tightly controlled experimental design in mice, which allowed the dissection of the relative contribution of the aforementioned factors to the visualization of markers of individual AHN stages. Fixation time emerged as the most prominent factor globally impeding the study of this process in mice. Moreover, the visualization of other particularly sensitive epitopes was further prevented by prolonged PMD. These results are crucial to disambiguate current controversies related to the occurrence of AHN not only in humans but also in other mammalian species. Significant impact of post-mortem time and fixation method on immunohistochemistry performance of antibody-based staining of common markers relevant to adult neurogenesis.
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Keywords

BornBrainsChildrenDoublecortinGenderGranule neuronsHippocampal neurogenesisLife on landMessenger-rnaMicrotubule-associated proteinProliferation

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Communications Biology due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2023, it was in position 13/109, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biology.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.38. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2026-04-05, the following number of citations:

  • WoS: 6
  • Scopus: 3
  • Open Alex: 4
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-05:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 33.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 33 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 19.
  • The number of mentions on the social network X (formerly Twitter): 33 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
Continuing with the social impact of the work, it is important to emphasize that, due to its content, it can be assigned to the area of interest of ODS 15 - Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss, with a probability of 58% according to the mBERT algorithm developed by Aurora University.
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Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Gallardo-Caballero, M) and Last Author (LLORENS MARTIN, MARIA VICTORIA).

the author responsible for correspondence tasks has been LLORENS MARTIN, MARIA VICTORIA.

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