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Analysis of institutional authors

Pazos, ManuelAuthor

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August 11, 2025
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Verrucomicrobium spinosum essential genome and the divergence of cell division in the PVC superphylum

Publicated to: Iscience. 28 (8): 113037- - 2025-08-15 28(8), DOI: 10.1016/j.isci.2025.113037

Authors:

Henriques, Valentina; Moyano-Palazuelo, David; Alonso-Pascual, Maria Teresa; Pazos, Manuel; Devos, Damien P; Rivas-Marin, Elena
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Affiliations

Autonomous Univ Madrid, Ctr Mol Biol Severo Ochoa UAM CSIC, Dept Mol Biol, Madrid, Spain - Author
Autonomous Univ Madrid, Lab Mass Spectrometry SIdI UAM, Madrid, Spain - Author
Pablo Olavide Univ, Ctr Andaluz Biologia Desarrollo CSIC, Seville, Spain - Author
Univ Lille, Inst Pasteur Lille, Ctr Infect & Immun Lille, CNRS,Inserm,CHU Lille,U1019 UMR 9017, F-59000 Lille, France - Author
Univ Seville, Fac Biol, Dept Genet, Seville, Spain - Author
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Abstract

The Planctomycetota-Chlamydiota-Verrucomicro biota superphylum comprises bacteria with divergent traits, epitomizing the vast biodiversity of non-model organisms that remains unexplored. Among its members, Planctomycetota and Chlamydiota exhibit unique adaptations, including variations in peptidoglycan structure and cell division without FtsZ. Verrucomicrobiota represents the only name-giving phylum within the PVC not extensively investigated. Here, exhaustive transposon mutagenesis combined with massive sequencing reveal the essential genome of Verrucomicrobium spinosum. The essentiality report demonstrates that the dcw cluster is essential except mraW. Additionally, peptidoglycan analysis evidences that the composition and saccular arrangement are canonical, contrarily to Planctomycetota and Chlamydiota. Targeted mutagenesis for V. spinosum was also developed, thus reinforcing its status as a model for Verrucomicrobiota. Ultimately, the evolutionary divergences of the dcw cluster in PVC, combined with the previous results, suggest that its Last Common Ancestor was a Gracilicutes-like bacterium dividing by an FtsZ-mediated binary fission with peptidoglycan, from which current phyla diverged.
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Keywords

AcetylatedBacteriaEscherichia-coliFtskGeneHighlightsMicrobial genomicsPeptidoglycanPhylogeneticPhylogeneticsProtein

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal iScience due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position 22/136, thus managing to position itself as a Q1 (Primer Cuartil), in the category Multidisciplinary Sciences.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-02:

  • WoS: 1
  • Scopus: 1
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-02:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 5.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 2 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 3.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: France.

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Awards linked to the item

FCT-Fundacao para a Ciencia e a Tecnologia, Portugal (2022.11400.BD) ; D. M.-P. by Junta de Andaluc & imath;a Predoctoral Grant (Predoc_00913) ; M.P. is an Atraccion de Talento M1 Fellow from Comunidad de Madrid (Spain) [2020-T1/BMD-19970] who receives support [PID2022-140818OA-I 00] from the Spanish Ministry of Science, Innovation and Universities (MICIU/AEI/https://doi.org/10.13039/501100011033 and ERDF/EU) ; D.P.D. and E.R.-M. by Mineco (Grant No. PID2020-119733GBI00) and the "Moore-Simons Project on the Origin of the Eukaryotic Cell" (Grant No. 9733/https://doi.org/10. 37807/GBMF9733) .
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