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Analysis of institutional authors

Ferrer, MercedesCorresponding Author

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November 25, 2024
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Effect of 5β-dihydrotestosterone on vasodilator function and on cell proliferation

Publicated to: PLoS ONE. 19 (10): e0312080- - 2024-10-23 19(10), DOI: 10.1371/journal.pone.0312080

Authors: Sanchez-Fernandez, David; Eguibar, Aritz; Lopez, Cristina; Cuesta, Angel M; Albinana, Virginia; Rogers-Ezewuike, Soline; Gomez-Rivas, Juan A; Saldana, Laura; Botella, Luisa M; Ferrer, Mercedes

Affiliations

CSIC, Ctr Invest Biol Margarita Salas, Dept Biomed Mol, Madrid, Spain - Author
Ctr Invest Biomed Red Bioingn Biomat & Nanomed, CIBER BBN, Madrid, Spain - Author
Hosp Clin San Carlos, Serv Urol, Madrid, Spain - Author
Hosp Quiron Salud, Serv Urol, Marbella, Spain - Author
Hosp Univ La Paz IdiPAZ, Grp Fisiopatol Osea & Biomat, Madrid, Spain - Author
Inst Salud Carlos III ISCIII, Ctr Invest Biomed Red Enfermedades Raras, CIBERER, Unidad 707, Madrid, Spain - Author
Univ Autonoma Madrid, Fac Med, Dept Fisiol, Madrid, Spain - Author
Univ Complutense Madrid, Fac Med, Dept Cirugia, Madrid, Spain - Author
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Abstract

Aging is one of the main factors associated with cardiovascular diseases. Androgens exert beneficial effects on the cardiovascular system and testosterone (TES) replacement therapy improves cardiometabolic risk factors. However, TES is contraindicated in patients with prostate cancer due to its proliferative effects on prostatic tumor cells. Additionally, TES and its reduced metabolites 5 alpha- and 5 beta-dihydrotestosterone (5 alpha-DHT and 5 beta-DHT) exert vasodilatory effects. Since androgen levels decrease during aging and 5 beta-DHT lacks genomic effects, this study is focused on analyzing its effect on vasodilator function and the proliferation rate of prostatic tumor and vascular smooth muscle cells. To study the vascular function, mesenteric arteries from aged-orchidectomized Sprague-Dawley rats were used. Mesenteric segments were divided into one control (without treatment) and three groups with the androgens (10 nM, 30 min) to analyze: acetylcholine- and sodium nitroprusside-induced responses and nitric oxide and superoxide anion production. To analyze cell proliferation, the effect of androgens on cell viability was determined. The results showed that 5 beta-DHT improves vasodilator function in arteries from aged-orchidectomized rats and induces antioxidant action, while the proliferation rate of the androgen-dependent prostatic tumor cells remains unaltered. These results make 5 beta-DHT a promising therapeutic agent for the treatment of cardiovascular pathologies.

Keywords

AcetylcholineAndrogenAndrogensAnimalsCardiovascular-diseasesCell proliferationDihydrotestosteroneEndothelial dysfunctionHumansMaleMenMesenteric arteriesMesenteric-arteriesMuscle, smooth, vascularNitric oxideNitric-oxideNitroprussideOrchiectomy increasesProstatic neoplasmsRatsRats, sprague-dawleyRisk-factorsSex-hormonesSuperoxidesTestosteroneVasodilationVasodilator agents

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal PLoS ONE due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Multidisciplinary.

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-08:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 6.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 6 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Sanchez-Fernandez, David) and Last Author (FERRER PARRA, MERCEDES).

the author responsible for correspondence tasks has been FERRER PARRA, MERCEDES.

Awards linked to the item

This study was supported by a grant from the Instituto de Salud Carlos III (PI19/01282) and Fondo Europeo de Desarrollo Regional to M. Ferrer, and a grant from the Ministry of Science and Innovation of Spain (PID2020-115371RB-I00) to L.M. Botella. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.