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This research was funded by the "Fondo de Investigaciones Sanitarias-ISCIII- FEDER", Ministry of Health, Spain (grant number PI23/00504), and co-funded by the European Regional Development Fund.

Analysis of institutional authors

Garcia-Olmo, DamianAuthorGarcia-Arranz, MarianoCorresponding Author

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October 21, 2024
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A Protocol for the Isolation of Oval Cells without Preconditioning

Publicated to:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 25 (19): 10497- - 2024-10-01 25(19), DOI: 10.3390/ijms251910497

Authors: Olivera-Salazar, Rocio; Sanchez, Aranzazu; Herrera, Blanca; Garcia-Saez, Juan; Vega-Clemente, Luz; Campos, Pedro Villarejo; Garcia-Olmo, Damian; Garcia-Arranz, Mariano

Affiliations

Biomed Res Networking Ctr Hepat & Digest Dis CIBER, Madrid 28029, Spain - Author
Complutense Univ Madrid UCM, Hlth Res Inst Hosp Clin San Carlos IdISSC, Fac Pharm, Dept Biochem & Mol Biol, Madrid 28040, Spain - Author
Fdn Jimenez Diaz Univ Hosp FJD, Dept Surg, Madrid 28040, Spain - Author
Fdn Jimenez Diaz Univ Hosp IIS FJD, Hlth Res Inst, New Therap Lab, Avda Reyes Catolicos 2, Madrid 28040, Spain - Author
Univ Autonoma Madrid, Dept Surg, Madrid, Spain - Author
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Abstract

Oval cells (OCs) is the name of hepatic progenitor cells (HPCs) in rodents. They are a small population of cells in the liver with the remarkable ability to proliferate and regenerate hepatocytes and cholangiocytes in response to acute liver damage. Isolating OCs generally requires a pretreatment with special diets, chemicals, and/or surgery to induce hepatic damage and OC proliferation in mice. Unfortunately, these pretreatments are not only painful for the mice but also increase the cost of the assays, and the effects on the different organs as well as on various liver cells are still unclear. Therefore, the search for a protocol to obtain OCs without prior liver damage is mandatory. In our study, we present a protocol to isolate murine OCs from healthy liver (HL-OCs) and compare them with OCs isolated from mice pretreated with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC-OCs). Our results demonstrated that cells derived from untreated mice exhibited similar behavior to those from treated mice in terms of surface marker expression, proliferation, and differentiation capacity. Therefore, given the impracticability of isolating human cells with prior hepatotoxic treatment, our model holds promise for enabling the isolation of progenitor cells from human tissue in the future. This advancement could prove invaluable for translational medicine in the understanding and treatment of liver diseases.

Keywords

3,5-diethoxycarbonyl-1,4-dihydrocollidineActivationAnimalsCell differentiationCell proliferationCell separationCells, culturedDifferentiationDifferentiation capacityHepatic progenitor cells (hpcs)HepatocytesHepatotoxic treatmentHumansLiverLiver diseaseLiver diseasesLiver progenitor cellsMaleMiceMice liver modelsMice, inbred c57blModelsOrigiOval cells (ocs)Pretreatment-free cell isolation protocolProliferationPyridinesRatRegenerationStem cellsStem-cell

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 68/231, thus managing to position itself as a Q1 (Primer Cuartil), in the category Chemistry, Multidisciplinary.

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-16:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 5 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

    It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

    • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

    Leadership analysis of institutional authors

    There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (GARCIA ARRANZ, MARIANO ANDRES).

    the author responsible for correspondence tasks has been GARCIA ARRANZ, MARIANO ANDRES.