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Grant support

Instituto de Salud Carlos III " co-funded by European Regional Development Fund (ERDF), "A way to make Europe" to A.M.B, Grant/Award Number: P118/00526; Universidad de Alcala cofunded by Banco Santander to R.H-L, Grant/Award Number: 572760

Analysis of institutional authors

Huertas-Larez, RaquelAuthor
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Article

Induction of more aggressive tumoral phenotypes in LNCaP and PC3 cells by serum exosomes from prostate cancer patients

Publicated to:INTERNATIONAL JOURNAL OF CANCER. 153 (10): 1829-1841 - 2023-08-01 153(10), DOI: 10.1002/ijc.34673

Authors: Huertas-Larez, Raquel; Munoz-Moreno, Laura; Recio-Aldavero, Jorge; Roman, Irene Dolores; Arenas, Maria Isabel; Blasco, Ana; Sanchis-Bonet, Angeles; Bajo, Ana M M

Affiliations

Hosp Univ Principe Asturias, Serv Anat Patol, Carretera Alcala Meco S-N, Alcala De Henares, Madrid, Spain - Author
Hosp Univ Principe Asturias, Serv Urol, Carretera Alcala Meco S-N, Alcala De Henares, Madrid, Spain - Author
Univ Alcala, Dept Biomed & Biotecnol, Unidad Biol Celular, Campus Cient Tecnol, Alcala De Henares, Madrid, Spain - Author
Univ Alcala, Fac Med & Ciencias Salud, Dept Biol Sistemas, Unidad Bioquim & Biol Mol, Campus Cient Tecnol, Alcala De Henares 28871, Madrid, Spain - Author
Univ Alcala, Grp Invest Canc Origen Epitelial, Alcala De Henares, Madrid, Spain - Author
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Abstract

Prostate cancer (PCa) is the second most frequent and sixth most fatal cancer in men worldwide. Despite its high prevalence, our understanding of its etiology and the molecular mechanisms involved in the progression of the disease is substantially limited. In recent years, the potential participation of exosomes in this process has been suggested. Therefore, we aim to study the effect of exosomes isolated from the serum of patients with PCa on various cellular processes associated with increased tumor aggressiveness in two PCa cell lines: LNCaP-FGC and PC3. The exosomes were isolated by filtration wand ultracentrifugation. Their presence was confirmed by immunodetection of specific markers and their size distribution was analyzed by Dynamic Light Scattering (DLS). The results obtained demonstrated that serum exosomes from PCa patients increased migration of PC3 cells and neuroendocrine differentiation of LNCaP-FGC cells regardless of the grade of the tumor. PCa serum exosomes also enhanced the secretion of enzymes related to invasiveness and resistance to chemotherapeutics, such as extracellular matrix metalloproteases 2 and 9, and gamma-glutamyltransferase in both cell lines. Altogether, these findings support the pivotal participation of exosomes released by tumoral cells in the progression of PCa. Future studies on the molecular mechanisms involved in the observed changes could provide crucial information on this disease and help in the discovery of new therapeutic targets.

Keywords
Cell line, tumorExosomesGamma-glutamyl-transferaseHumansKappa-bLncap-fgcMaleOverexpressioPc-3 cellsPc3PhenotypeProstate cancerProstatic neoplasmsSerum exosomesTumor progressioTumor progression

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal INTERNATIONAL JOURNAL OF CANCER due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2023, it was in position 54/322, thus managing to position itself as a Q1 (Primer Cuartil), in the category Oncology.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 1.28, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions May 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-05-07, the following number of citations:

  • Scopus: 2
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-07:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 4.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 4 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 3.
  • The number of mentions on the social network X (formerly Twitter): 6 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (HUERTAS LAREZ, RAQUEL) .