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Analysis of institutional authors

Espinosa, EnriqueAuthorGonzalez-Peramato, PilarAuthorPinto-Marin, AlvaroAuthorGamez-Pozo, AngeloCorresponding Author

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January 23, 2023
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Article

Proteomics Characterization of Clear Cell Renal Cell Carcinoma

Publicated to: Journal Of Clinical Medicine. 12 (1): 384- - 2023-01-01 12(1), DOI: 10.3390/jcm12010384

Authors:

Miranda-Poma, J; Trilla-Fuertes, L; López-Vacas, R; López-Camacho, E; García-Fernández, E; Pertejo, A; Lumbreras-Herrera, M; Zapater-Moros, A; Díaz-Almirón, M; Dittmann, A; Vara, JAF; Espinosa, E; González-Peramato, P; Pinto-Marín, A; Gámez-Pozo, A
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Affiliations

Biomed Mol Med SL, Madrid 28049, Spain - Author
Funct Genom Ctr Zurich, CH-8057 Zurich, Switzerland - Author
Hosp Univ La Paz IdiPAZ, Biostat Unit, Madrid 28046, Spain - Author
Hosp Univ La Paz IdiPAZ, Mol Oncol Lab, Madrid 28046, Spain - Author
Hosp Univ La Paz, Med Oncol Serv, Madrid 28046, Spain - Author
Hosp Univ Quironsalud Madrid, Med Oncol Serv, Madrid 28223, Spain - Author
ISCIII, Biomed Res Networking Ctr Oncol CIBERONC, Madrid 28029, Spain - Author
Univ Autonoma Madrid, Catedra UAM Amgen, Madrid 28049, Spain - Author
Univ Hosp La Paz, Dept Pathol, Madrid 28046, Spain - Author
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Abstract

Purpose: To explore the tumor proteome of patients diagnosed with localized clear cell renal cancer (ccRCC) and treated with surgery. Material and methods: A total of 165 FFPE tumor samples from patients diagnosed with ccRCC were analyzed using DIA-proteomics. Proteomics ccRCC subtypes were defined using a consensus cluster algorithm (CCA) and characterized by a functional approach using probabilistic graphical models and survival analyses. Results: We identified and quantified 3091 proteins, including 2026 high-confidence proteins. Two proteomics subtypes of ccRCC (CC1 and CC2) were identified by CC using the high-confidence proteins only. Characterization of molecular differences between CC1 and CC2 was performed in two steps. First, we defined 514 proteins showing differential expression between the two subtypes using a significance analysis of microarrays analysis. Proteins overexpressed in CC1 were mainly related to translation and ribosome, while proteins overexpressed in CC2 were mainly related to focal adhesion and membrane. Second, a functional analysis using probabilistic graphical models was performed. CC1 subtype is characterized by an increased expression of proteins related to glycolysis, mitochondria, translation, adhesion proteins related to cytoskeleton and actin, nucleosome, and spliceosome, while CC2 subtype showed higher expression of proteins involved in focal adhesion, extracellular matrix, and collagen organization. Conclusions: ccRCC tumors can be classified in two different proteomics subtypes. CC1 and CC2 present specific proteomics profiles, reflecting alterations of different molecular pathways in each subtype. The knowledge generated in this type of studies could help in the development of new drugs targeting subtype-specific deregulated pathways.
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Keywords

clear cell renal cell carcinomamolecular subtypesClass discoveryClear cell renal cell carcinomaExpressionManagementMolecular subtypesNephrectomyProteomicsRevealsTherapy

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Journal of Clinical Medicine due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2023, it was in position 59/329, thus managing to position itself as a Q1 (Primer Cuartil), in the category Medicine, General & Internal.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.15. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2026-04-05, the following number of citations:

  • WoS: 4
  • Scopus: 5
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-05:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 7.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 7 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 4.
  • The number of mentions on the social network X (formerly Twitter): 6 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://repositorio.uam.es/handle/10486/719136
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Switzerland.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (GAMEZ POZO, ANGELO).

the author responsible for correspondence tasks has been GAMEZ POZO, ANGELO.

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