Administration of anti-inflammatory cytokines is a common therapeutic strategy in chronic inflammatory diseases. However, these treatments have several limitations that can be overcome by genetic delivery. Gene therapy is an efficient method for delivering therapeutic molecules to target cells. Development of gene therapy approaches for treating chronic inflammatory diseases is a challenge as it requires the production of anti-inflammatory molecules at the diseased tissues only when they are needed. In our research group we have generated several inflammation-regulated lentiviral expression systems which are induced upon acute inflammation. Our aim is to express anti-inflammatory molecules under these inflammation-inducible systems in cell cultures and in animal models. These results may help in the design of new strategies for the local treatment of chronic inflammatory diseases such as cardiovascular diseases and autoimmune disorders.